|
| Status |
Public on Jul 31, 2008 |
| Title |
B16 vs melan-a, rep 3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
B16 cells
|
| Organism |
Mus musculus |
| Characteristics |
Growth medium: DMEM
Strain: C57/Bl6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using tri-reagent following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Invitrogen Superscript cDNA direct labeling system
|
| |
|
| Channel 2 |
| Source name |
melan-a cells
|
| Organism |
Mus musculus |
| Characteristics |
Growth medium: RPMI 1640
Strain: C57/Bl6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using tri-reagent following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Invitrogen Superscript cDNA direct labeling system
|
| |
|
| |
| Hybridization protocol |
Used Agilent hybridization solution and Biomicrosystem MAUI hybridization station, both according to manufacturers' instructions. Hybridization was for 16hr at 60C.
|
| Scan protocol |
Scanned on an Perkin Elmer ScanArray Express scanner.
|
| Description |
B16 vs melan-a, replicate 3 of 6; standard labeling
|
| Data processing |
LOWESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal. Imagene software (Biodiscovery) was used.
|
| |
|
| Submission date |
May 28, 2008 |
| Last update date |
Jun 20, 2008 |
| Contact name |
James Denvir |
| E-mail(s) |
denvir@marshall.edu
|
| Organization name |
Marshall University School of Medicine
|
| Department |
Biochemistry and Microbiology
|
| Lab |
Genomics and Bioinformatics Core Facility
|
| Street address |
One John Marshall Drive
|
| City |
Huntington |
| State/province |
WV |
| ZIP/Postal code |
25755 |
| Country |
USA |
| |
|
| Platform ID |
GPL2872 |
| Series (2) |
| GSE11584 |
Melan-a mouse melanocytes vs. B16 mouse melanoma cells |
| GSE11588 |
Melan-a melanocytes and B16 melanoma cells |
|
