|
| Status |
Public on Nov 06, 2018 |
| Title |
sCD74_161027_8h Rep. 1 |
| Sample type |
RNA |
| |
|
| Source name |
cardiac fibroblasts treated for 8 hrs with 40 nM sCD74
|
| Organism |
Mus musculus |
| Characteristics |
tissue: heart
age: 1-7 days post natal
genetic background: C57BL/6j
genotype: wildtype
cell type: cardiac fibroblasts
|
| Treatment protocol |
Myofibroblasts were solitarily as well as co-treated with either 8nM MIF or 40nM sCD74 for 8 hours.
|
| Growth protocol |
Cardiac fibroblasts were isolated from hearts of neonatal wildtype mice (C57BL/6J) and maintained for 10 to 14 days in culture, where they fully differentiated into myofibroblasts.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After 8 hours of stimulation, supernatant was removed and total RNA was isolated using the Nucleo Spin RNA/Protein kit (Machery-Nagel, Düren, Germany).
|
| Label |
biotin
|
| Label protocol |
Fragmented, biotinylated cDNAs were prepared from 150 ng total RNA using the Affymetrix WT plus reagent kit according to the manufacturers instructions.
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix haybridization kit materials according to the manufacturers instructions: hybridization 45°C for 16 hours at 60 rpm; Fluidics Station Program FS_450_0007
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Data processing |
Raw image data were analysed with Affymetrix® Expression Console™ Software (Affymetrix, USA), and gene expression intensities were normalized and summarized with SST robust multiarray average algorithm (Affymetrix): SST-RMA. Further analyses was performed using the Transcriptome Analysis Console (TAC) Software of Affymetrix.
|
| |
|
| Submission date |
Jan 10, 2018 |
| Last update date |
Nov 06, 2018 |
| Contact name |
Bernd Denecke |
| E-mail(s) |
bernd.denecke@rwth-aachen.de
|
| Phone |
+49 241 8089918
|
| Organization name |
RWTH Aachen University
|
| Department |
IZKF Aachen
|
| Street address |
Pauwelsstrasse 30
|
| City |
Aachen |
| State/province |
NRW |
| ZIP/Postal code |
52074 |
| Country |
Germany |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE108999 |
The effect of soluble CD74 (sCD74) and recombinant macrophage migration inhibitory factor (MIF) treatment on the gene expression profile in cardiac myofibroblasts |
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