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Status |
Public on Nov 06, 2018 |
Title |
sCD74_161208_8h Rep. 3 |
Sample type |
RNA |
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Source name |
cardiac fibroblasts treated for 8 hrs with 40 nM sCD74
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Organism |
Mus musculus |
Characteristics |
tissue: heart age: 1-7 days post natal genetic background: C57BL/6j genotype: wildtype cell type: cardiac fibroblasts
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Treatment protocol |
Myofibroblasts were solitarily as well as co-treated with either 8nM MIF or 40nM sCD74 for 8 hours.
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Growth protocol |
Cardiac fibroblasts were isolated from hearts of neonatal wildtype mice (C57BL/6J) and maintained for 10 to 14 days in culture, where they fully differentiated into myofibroblasts.
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Extracted molecule |
total RNA |
Extraction protocol |
After 8 hours of stimulation, supernatant was removed and total RNA was isolated using the Nucleo Spin RNA/Protein kit (Machery-Nagel, Düren, Germany).
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Label |
biotin
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Label protocol |
Fragmented, biotinylated cDNAs were prepared from 150 ng total RNA using the Affymetrix WT plus reagent kit according to the manufacturers instructions.
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Hybridization protocol |
Samples were hybridized using Affymetrix haybridization kit materials according to the manufacturers instructions: hybridization 45°C for 16 hours at 60 rpm; Fluidics Station Program FS_450_0007
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Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
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Data processing |
Raw image data were analysed with Affymetrix® Expression Console™ Software (Affymetrix, USA), and gene expression intensities were normalized and summarized with SST robust multiarray average algorithm (Affymetrix): SST-RMA. Further analyses was performed using the Transcriptome Analysis Console (TAC) Software of Affymetrix.
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Submission date |
Jan 10, 2018 |
Last update date |
Nov 06, 2018 |
Contact name |
Bernd Denecke |
E-mail(s) |
bernd.denecke@rwth-aachen.de
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Phone |
+49 241 8089918
|
Organization name |
RWTH Aachen University
|
Department |
IZKF Aachen
|
Street address |
Pauwelsstrasse 30
|
City |
Aachen |
State/province |
NRW |
ZIP/Postal code |
52074 |
Country |
Germany |
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Platform ID |
GPL23038 |
Series (1) |
GSE108999 |
The effect of soluble CD74 (sCD74) and recombinant macrophage migration inhibitory factor (MIF) treatment on the gene expression profile in cardiac myofibroblasts |
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