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Sample GSM293171 Query DataSets for GSM293171
Status Public on Oct 27, 2008
Title Btk knock out DT40, FCS batch 2; B7.10 2 C6
Sample type RNA
 
Source name B7.10 cells grown for 6 hours without stimulation
Organism Gallus gallus
Characteristics Genotype: Btk KO DT40 without endogenous Btk
Treatment protocol All the cell lines were stimulated with IgM (M4)
Growth protocol DT40 and B7.10 cells were cultivated in three different fetal calf serum batches, while B41-13 and B46-5 were grown in a single serum batch. Both unstimulated and stimulated cells were grown in the same way, starting in 1 ml serum-free medium for 15 min at 37°C with 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with RNeasy Mini Kit (Qiagen, Valencia, CA, USA).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Genechip Scanner 30007G.
Description Gene expression data from Btk KO chicken DT40 cell line grown for 6 hours in fetal calf serum batch 2 without any stimulation
Data processing RMA
 
Submission date May 29, 2008
Last update date Oct 27, 2008
Contact name Hossain Nawaz
E-mail(s) hossain.nawaz@ki.se
Phone +46858583806
Fax +46858583650
Organization name Karolinska Institutet
Department LABMED
Lab KFC/MCG Lab 514
Street address Hälsovägen 7
City Huddinge
State/province Stockholm
ZIP/Postal code 14186
Country Sweden
 
Platform ID GPL3213
Series (1)
GSE11597 Expression data from chicken B cell lymphoma (DT40) cell lines.

Data table header descriptions
ID_REF
VALUE Quantification

Data table
ID_REF VALUE
AFFX-BioB-5_at 7.08238
AFFX-BioB-M_at 7.53277
AFFX-BioB-3_at 7.34475
AFFX-BioC-5_at 8.53488
AFFX-BioC-3_at 8.38991
AFFX-BioDn-5_at 9.86281
AFFX-BioDn-3_at 10.7552
AFFX-CreX-5_at 12.3812
AFFX-CreX-3_at 12.7204
AFFX-DapX-5_at 7.50692
AFFX-DapX-M_at 8.60259
AFFX-DapX-3_at 9.14125
AFFX-LysX-5_at 4.47761
AFFX-LysX-M_at 4.89604
AFFX-LysX-3_at 6.80004
AFFX-PheX-5_at 5.78675
AFFX-PheX-M_at 6.07417
AFFX-PheX-3_at 6.6222
AFFX-ThrX-5_at 6.56084
AFFX-ThrX-M_at 6.37067

Total number of rows: 38535

Table truncated, full table size 1040 Kbytes.




Supplementary file Size Download File type/resource
GSM293171.CEL.gz 3.2 Mb (ftp)(http) CEL
GSM293171.chp.gz 328.3 Kb (ftp)(http) CHP
GSM293171.dcp.gz 2.7 Mb (ftp)(http) DCP
Processed data included within Sample table

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