|
| Status |
Public on Oct 27, 2008 |
| Title |
Btk knock out DT40 reconstituted with kinase inactive human BTK , FCS batch 1; B46-5 1 S2 |
| Sample type |
RNA |
| |
|
| Source name |
B46-5 cells grown for 2 hours after stimulation with IgM
|
| Organism |
Gallus gallus |
| Characteristics |
Genotype: Btk KO DT40 reconstituted with kinase inactive human BTK
|
| Treatment protocol |
All the cell lines were stimulated with IgM (M4)
|
| Growth protocol |
DT40 and B7.10 cells were cultivated in three different fetal calf serum batches, while B41-13 and B46-5 were grown in a single serum batch. Both unstimulated and stimulated cells were grown in the same way, starting in 1 ml serum-free medium for 15 min at 37°C with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with RNeasy Mini Kit (Qiagen, Valencia, CA, USA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Genechip Scanner 30007G.
|
| Description |
Gene expression data from chicken B46-5 cell line grown for 2 hours in fetal calf serum batch 1 after stimulation with IgM
|
| Data processing |
RMA
|
| |
|
| Submission date |
May 29, 2008 |
| Last update date |
Oct 27, 2008 |
| Contact name |
Hossain Nawaz |
| E-mail(s) |
hossain.nawaz@ki.se
|
| Phone |
+46858583806
|
| Fax |
+46858583650
|
| Organization name |
Karolinska Institutet
|
| Department |
LABMED
|
| Lab |
KFC/MCG Lab 514
|
| Street address |
Hälsovägen 7
|
| City |
Huddinge |
| State/province |
Stockholm |
| ZIP/Postal code |
14186 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE11597 |
Expression data from chicken B cell lymphoma (DT40) cell lines. |
|
