|
Status |
Public on Dec 01, 2018 |
Title |
Brain_DEREG_sham_Day14 |
Sample type |
RNA |
|
|
Source name |
Brain tissue from sham DEREG mice
|
Organism |
Mus musculus |
Characteristics |
tissue: Brain cell: whole tissue genotype/variation: DEREG
|
Treatment protocol |
For brain tissue, total RNA was isolated using QIAzol Lysis Reagent (QIAGEN) from the day 14 post-ischemic or sham brains of WT or DEREG mice (slice 3 on the ischemic side). For CD4+ T cells, total RNA was isolated using a TRIzolTM LS reagent (Invitrogen) from the CD4+ T cells in WT ischemic mice brain or in untreated mice spleen.
|
Extracted molecule |
total RNA |
Extraction protocol |
For brain tissue, total RNA was isolated using QIAzol Lysis Reagent (QIAGEN) from the day 14 post-ischemic or sham brains of WT or DEREG mice (slice 3 on the ischemic side). For CD4+ T cells, total RNA was isolated using a TRIzolTM LS reagent (Invitrogen) from the CD4+ T cells in WT ischemic mice brain or in untreated mice spleen.
|
Label |
Cy3
|
Label protocol |
One-Color RNA Spike-In Kit, Low Input Quick Amp Labeling Kit (Agilent)
|
|
|
Hybridization protocol |
Expression Hybridization Kit (Agilent)
|
Scan protocol |
DNA Microarray Scanner (2505C, Agilent), Gene Expression Wash Pack (Agilent)
|
Data processing |
Feature Extraction ver 10.7.1.1 (Agielnt)
|
|
|
Submission date |
Jan 16, 2018 |
Last update date |
Dec 02, 2018 |
Contact name |
Minako Ito |
E-mail(s) |
minakoito@bioreg.kyushu-u.ac.jp
|
Organization name |
Kyushu University
|
Street address |
3-1-1 Maidashi, Higashi-ku
|
City |
Fukuoka |
ZIP/Postal code |
812-8582 |
Country |
Japan |
|
|
Platform ID |
GPL21163 |
Series (1) |
GSE109259 |
Expression data in ischemic brain tissue and brain Tregs |
|