|
| Status |
Public on Jan 02, 2019 |
| Title |
ingWAT Gipr-/- /S100a9-/- BM; TF73 |
| Sample type |
RNA |
| |
|
| Source name |
ingWAT Gipr-/- /Protein S100-A9 (S100 Calcium Binding Protein A9) -/- bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Gipr-/- /S100a9-/-
tissue: Inguinal subcutaneous fat
strain: C57BL/6J
gender: male
|
| Treatment protocol |
The fat tissue were isolated from chimeric mice reconstituted with bone marrow from Gipr-/-, S100a9-/- and Gipr-/-/ S100a9-/- mice.
|
| Growth protocol |
Epididymal fat pads or inguinal subcutaneous fat were surgically removed from the following BM chimera mice after 6 weeks of BM reconstitution and additional 14 weeks of HFD (60% cal from fat):WT into WT, Gipr-/- into WT, Gipr-/-S100a9-/-.Fat tissues were frozen in liquid nitrogen and kept at -80 degree C until RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using QIAzol Lysis Reagent and Lipid RNA extraction RNeasy Mini Kit. RNA purity was assessed with NanoDrop™ (2000/2000c Spectrophotometers; Thermo, Fisher Scientific) and TapeStation Analysis (Software A.02.01, Agilent Technologies). RNA concentrations were determined by the absorbance at 260 nm and quality control standards were A260/A280 = 1.8 – 2.0.
|
| Label |
biotin
|
| Label protocol |
Samples were prepared according to the standard Affymetrix WT PLUS Reagent Kit protocol and Affymetrix WT Terminal Labeling Kit from 100 ng total RNA starting material
|
| |
|
| Hybridization protocol |
GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to the standard GeneChip Expression Wash, Stain and Scan protocol.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 3000 7G scanner according to the instruction manual.
|
| Description |
C57BL/6J mice (Envigo)
|
| Data processing |
RNA normalization, summarization by mean, all were done by Partek Genomics suite (version 6.6)).
|
| |
|
| Submission date |
Jan 18, 2018 |
| Last update date |
Jan 02, 2019 |
| Contact name |
Metsada Pasmanik-Chor |
| E-mail(s) |
metsada@tauex.tau.ac.il
|
| Organization name |
Tel Aviv University
|
| Department |
Biology
|
| Lab |
Bioinformatics Unit
|
| Street address |
Ramat Aviv
|
| City |
Tel Aviv |
| ZIP/Postal code |
69978 |
| Country |
Israel |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE109371 |
Glucose-dependent insulinotropic polypeptide ameliorates obesity and insulin resistance by attenuating S100A8/9 in myeloid cells |
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