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Status |
Public on Jan 02, 2019 |
Title |
epiWAT Gipr-/- /S100a9-/- BM; TF60 |
Sample type |
RNA |
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|
Source name |
epiWAT Gipr-/- /Protein S100-A9 (S100 Calcium Binding Protein A9) -/- bone marrow
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Organism |
Mus musculus |
Characteristics |
genotype: Gipr-/- /S100a9-/- tissue: Epididymal fat pads strain: C57BL/6J gender: male
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Treatment protocol |
The fat tissue were isolated from chimeric mice reconstituted with bone marrow from Gipr-/-, S100a9-/- and Gipr-/-/ S100a9-/- mice.
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Growth protocol |
Epididymal fat pads or inguinal subcutaneous fat were surgically removed from the following BM chimera mice after 6 weeks of BM reconstitution and additional 14 weeks of HFD (60% cal from fat):WT into WT, Gipr-/- into WT, Gipr-/-S100a9-/-.Fat tissues were frozen in liquid nitrogen and kept at -80 degree C until RNA extraction.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was prepared using QIAzol Lysis Reagent and Lipid RNA extraction RNeasy Mini Kit. RNA purity was assessed with NanoDrop™ (2000/2000c Spectrophotometers; Thermo, Fisher Scientific) and TapeStation Analysis (Software A.02.01, Agilent Technologies). RNA concentrations were determined by the absorbance at 260 nm and quality control standards were A260/A280 = 1.8 – 2.0.
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Label |
biotin
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Label protocol |
Samples were prepared according to the standard Affymetrix WT PLUS Reagent Kit protocol and Affymetrix WT Terminal Labeling Kit from 100 ng total RNA starting material
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|
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Hybridization protocol |
GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to the standard GeneChip Expression Wash, Stain and Scan protocol.
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Scan protocol |
GeneChips were scanned using the Affymetrix 3000 7G scanner according to the instruction manual.
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Description |
C57BL/6J mice (Envigo)
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Data processing |
RNA normalization, summarization by mean, all were done by Partek Genomics suite (version 6.6)).
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Submission date |
Jan 18, 2018 |
Last update date |
Jan 02, 2019 |
Contact name |
Metsada Pasmanik-Chor |
E-mail(s) |
metsada@tauex.tau.ac.il
|
Organization name |
Tel Aviv University
|
Department |
Biology
|
Lab |
Bioinformatics Unit
|
Street address |
Ramat Aviv
|
City |
Tel Aviv |
ZIP/Postal code |
69978 |
Country |
Israel |
|
|
Platform ID |
GPL23038 |
Series (1) |
GSE109371 |
Glucose-dependent insulinotropic polypeptide ameliorates obesity and insulin resistance by attenuating S100A8/9 in myeloid cells |
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