|
| Status |
Public on Aug 06, 2018 |
| Title |
adult hMYC-2_ALL_sample-3 |
| Sample type |
RNA |
| |
|
| Source name |
GFP+_FACS purified ALL from total body
|
| Organism |
Danio rerio |
| Characteristics |
tissue: Leukemia
genetic background: rag2:hMYC;lck:eGFP double transgenic line
cell type: GFP+_FACS purified ALL
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol starting from 100 ng of total RNA (GeneChip® 3’ IVT Express Kit Technical Manual, 2008, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Zebrafish Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000
|
| Description |
Gene expression data
|
| Data processing |
Microarray data (.CEL files) were normalized using the justRMA algorithm and analyzed using R-Bioconductor (Version 2.15.3)
|
| |
|
| Submission date |
Jan 19, 2018 |
| Last update date |
Aug 06, 2018 |
| Contact name |
Chiara Borga |
| E-mail(s) |
chiaraborga@gmail.com
|
| Organization name |
University of Padua
|
| Street address |
Corso Stati Uniti,4
|
| City |
Padova |
| ZIP/Postal code |
35127 |
| Country |
Italy |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE109437 |
Evidences of a MYC-driven precursor-B cell acute lymphoblastic leukemia (ALL) arising in the zebrafish rag2:hMYC T-cell ALL model |
|
