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Sample GSM294929 Query DataSets for GSM294929
Status Public on May 20, 2009
Title Rat_Vagina_E2_5Days_rep1
Sample type RNA
 
Source name Vaginal tissue from OVX rats treated 20ug/kg 17estradiol for 5 days
Organism Rattus norvegicus
Characteristics Rat_Vagina_E2_5Days_rep1
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA followed by Qiagen RNAeasy purification was performed according to the manufacturer's instructions
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip RAE230B arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description 230bB4E
Data processing The data were analyzed with GCOS1.0 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date May 30, 2008
Last update date May 20, 2009
Contact name Scott Jelinsky
E-mail(s) Scott.Jelinsky@pfizer.com
Phone 617-674-7272
Organization name Pfizer
Department Inflammation and Immunology
Lab Computational Precision Medicine
Street address 610 Main Street
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
 
Platform ID GPL342
Series (1)
GSE11622 Molecular Analysis of the Vaginal Response to Estrogens in the Ovariectomized Rat and Postmenopausal Woman

Data table header descriptions
ID_REF
VALUE GCOS signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 3877.205
AFFX-BioB-M_at 15279.48
AFFX-BioB-3_at 15958.74
AFFX-BioC-5_at 640.9156
AFFX-BioC-3_at 1178.933
AFFX-BioDn-5_at 3.09725
AFFX-BioDn-3_at 16173.83
AFFX-CreX-5_at 3727.346
AFFX-CreX-3_at 1188.383
AFFX-DapX-5_at 393.1847
AFFX-DapX-M_at 826.7723
AFFX-DapX-3_at 342.4842
AFFX-LysX-5_at 35.34486
AFFX-LysX-M_at 10.42225
AFFX-LysX-3_at 17.03648
AFFX-PheX-5_at 3.471521
AFFX-PheX-M_at 10.53994
AFFX-PheX-3_at 12.62607
AFFX-ThrX-5_at 5.37942
AFFX-ThrX-M_at 7.913936

Total number of rows: 15333

Table truncated, full table size 299 Kbytes.




Supplementary file Size Download File type/resource
GSM294929.cel.gz 2.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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