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Sample GSM294936 Query DataSets for GSM294936
Status Public on May 20, 2009
Title Rat_Vagina_Veh_3Days_rep3
Sample type RNA
 
Source name Vaginal tissue from OVX rats treated with vehicle for 3 days
Organism Rattus norvegicus
Characteristics Rat_Vagina_Veh_3Days_rep3
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA followed by Qiagen RNAeasy purification was performed according to the manufacturer's instructions
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip RAE230B arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description 230bC5V
Data processing The data were analyzed with GCOS1.0 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date May 30, 2008
Last update date May 20, 2009
Contact name Scott Jelinsky
E-mail(s) Scott.Jelinsky@pfizer.com
Phone 617-674-7272
Organization name Pfizer
Department Inflammation and Immunology
Lab Computational Precision Medicine
Street address 610 Main Street
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
 
Platform ID GPL342
Series (1)
GSE11622 Molecular Analysis of the Vaginal Response to Estrogens in the Ovariectomized Rat and Postmenopausal Woman

Data table header descriptions
ID_REF
VALUE GCOS signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 1024.12
AFFX-BioB-M_at 4246.593
AFFX-BioB-3_at 4229.408
AFFX-BioC-5_at 160.1926
AFFX-BioC-3_at 344.7958
AFFX-BioDn-5_at 1.883575
AFFX-BioDn-3_at 4961.812
AFFX-CreX-5_at 1238.84
AFFX-CreX-3_at 334.7258
AFFX-DapX-5_at 124.7359
AFFX-DapX-M_at 253.0776
AFFX-DapX-3_at 85.92305
AFFX-LysX-5_at 3.045957
AFFX-LysX-M_at 15.84771
AFFX-LysX-3_at 7.884379
AFFX-PheX-5_at 1.639654
AFFX-PheX-M_at 1.702134
AFFX-PheX-3_at 18.842
AFFX-ThrX-5_at 1.818864
AFFX-ThrX-M_at 2.84027

Total number of rows: 15333

Table truncated, full table size 299 Kbytes.




Supplementary file Size Download File type/resource
GSM294936.cel.gz 2.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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