|
| Status |
Public on Nov 17, 2009 |
| Title |
CAM E10 replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
chick embryo (day10) chorioallantoic membrane (CAM), replicate 3
|
| Organism |
Gallus gallus |
| Characteristics |
CAMs were isolated from normal embryos at developmental day E10 (=HH>35)
|
| Biomaterial provider |
Martin Hagedorn
|
| Growth protocol |
Chick embryos were incubated at 37.8ºC and 75% humidity.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
CAMs were immediately snap-frozen in liquid nitrogen after excision. Total RNA was isolated using RNeasy mini kits (Qiagen) according to the manufacturer's instructions and quantified using a NanoDrop spectrophotometer.
|
| Label |
biotin
|
| Label protocol |
Complementary RNA (cRNA) was prepared according to the manufacturer’s (Affymetrix) 'standard protocol'.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized for 16 hr at 45C on the GeneChip Chicken Genome array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
We investigated vascular development and maturation in vivo by determining global gene regulation throughout the formation of the chick chorio-allantoic membrane (CAM).
|
| Data processing |
Data were analyzed with the GCOS 1.2 software (Affymetrix), using the default analysis settings and global scaling as normalization method, with a trimmed mean target intensity value (TGT) of each array arbitrarily set to 100.
|
| |
|
| Submission date |
Jun 02, 2008 |
| Last update date |
Nov 08, 2009 |
| Contact name |
John De Vos |
| E-mail(s) |
john.devos@inserm.fr
|
| Organization name |
University Hospital of Montpellier
|
| Department |
Institute for Research in Biotherapy
|
| Lab |
Microarray Core Facility
|
| Street address |
80 av. Augustin Fliche
|
| City |
Montpellier |
| ZIP/Postal code |
34000 |
| Country |
France |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE11636 |
Correlating global gene regulation to morphogenesis and maturation in the chick extra-embryonic vascular system |
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