|
| Status |
Public on Jun 05, 2009 |
| Title |
DU145_preNeg_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
negative control
|
| Organism |
Homo sapiens |
| Characteristics |
DU145 cells
transfected with Pre-miR™ miRNA Precursor Molecule negative control #1(Ambion)
|
| Treatment protocol |
Transfection with 20nM Pre-miR™ miRNA Precursor Molecules (Ambion), using Lipofectamine2000 (Invitrogen).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Three days after transfection, cells were harvested and total RNA extracted using Trizol Reagent (Invitrogen) according to the manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
standard Illumina protocol (Cy3)
|
| |
|
| Hybridization protocol |
standard Illumina protocol
|
| Scan protocol |
standard Illumina protocol
|
| Description |
DU145 cells transfected with Pre-miR™ miRNA Precursor Molecule negative control #1(Ambion)
|
| Data processing |
BeadStudio Gene Expression Module v_3; cubic spline normalization; filtering: detection p-value<0.05
|
| |
|
| Submission date |
Jun 06, 2008 |
| Last update date |
Jun 06, 2008 |
| Contact name |
Paolo Gandellini |
| E-mail(s) |
paolo.gandellini@istitutotumori.mi.it
|
| Organization name |
Fondazione IRCCS Istituto Nazionale Tumori
|
| Department |
Department of Applied Research and Technological Development
|
| Street address |
Via Amadeo 42
|
| City |
Milan |
| ZIP/Postal code |
20133 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6104 |
| Series (1) |
| GSE11701 |
Genes modulated by miR-205 in DU145 prostate cancer cells |
|
