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Sample GSM297217 Query DataSets for GSM297217
Status Public on Jun 05, 2009
Title DU145_preNeg_rep4
Sample type RNA
 
Source name negative control
Organism Homo sapiens
Characteristics DU145 cells
transfected with Pre-miR™ miRNA Precursor Molecule negative control #1(Ambion)
Treatment protocol Transfection with 20nM Pre-miR™ miRNA Precursor Molecules (Ambion), using Lipofectamine2000 (Invitrogen).
Extracted molecule total RNA
Extraction protocol Three days after transfection, cells were harvested and total RNA extracted using Trizol Reagent (Invitrogen) according to the manufacturer's instructions
Label Cy3
Label protocol standard Illumina protocol (Cy3)
 
Hybridization protocol standard Illumina protocol
Scan protocol standard Illumina protocol
Description DU145 cells transfected with Pre-miR™ miRNA Precursor Molecule negative control #1(Ambion)
Data processing BeadStudio Gene Expression Module v_3; cubic spline normalization; filtering: detection p-value<0.05
 
Submission date Jun 06, 2008
Last update date Jun 06, 2008
Contact name Paolo Gandellini
E-mail(s) paolo.gandellini@istitutotumori.mi.it
Organization name Fondazione IRCCS Istituto Nazionale Tumori
Department Department of Applied Research and Technological Development
Street address Via Amadeo 42
City Milan
ZIP/Postal code 20133
Country Italy
 
Platform ID GPL6104
Series (1)
GSE11701 Genes modulated by miR-205 in DU145 prostate cancer cells

Data table header descriptions
ID_REF
VALUE normalized signal intensity

Data table
ID_REF VALUE
ILMN_1725528 164.5
ILMN_1746533 37.5
ILMN_1800540 1046.4
ILMN_1785340 68.6
ILMN_1779536 45.2
ILMN_1654552 420.9
ILMN_1654861 296.2
ILMN_1697268 821.6
ILMN_1720430 61.4
ILMN_1665877 948.4
ILMN_1789751 676.5
ILMN_1695763 119.9
ILMN_1751530 171.5
ILMN_1763386 614.5
ILMN_1730670 104.4
ILMN_1660840 1240.6
ILMN_1805175 312.9
ILMN_1688299 48.4
ILMN_1762255 111.6
ILMN_1751124 68.1

Total number of rows: 11677

Table truncated, full table size 211 Kbytes.




Supplementary file Size Download File type/resource
GSM297217.txt.gz 305.7 Kb (ftp)(http) TXT
Processed data included within Sample table

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