Tri-Reagent/BCP ( Molecular Research Center, Cincinnati, OH ). Cleanup with Qiagen ( Valencia, CA ) Rneasy final step
Label
Alexa-647 Streptavidin
Label protocol
Biotin-labeled cRNA was prepared by linear amplification of the Poly(A)+ RNA population within the total RNA sample
Hybridization protocol
Arrays were hybridized at 37C for 18h in a shaking incubator.
Scan protocol
Arrays were scanned with Axon GenePixPro 4000B (Molecular Devices, Axon Instruments, Union City, CA).
Description
Retina, experimental sample for 3 month post streptozotocin injection experiment
Data processing
Raw values represent the final number of the spot after background is subtracted out. Normalized values represent median global normalization method, i.e. raw intensity/median intensity of all spots on the array
