|
| Status |
Public on Jul 11, 2008 |
| Title |
Gene expression profile of MEF cells transformed by Ras + Twist1 |
| Sample type |
RNA |
| |
|
| Source name |
Primary MEF cells transformed by the two H-RasV12 + Twist1 oncogènes
|
| Organism |
Mus musculus |
| Characteristics |
Primary fibroblasts from C57/B16 x DBA2 F1 mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA form primary or Ras + Twist-transformed MEF cells were prepared in duplicates using an RNeasy Mini kit (Quiagen).
|
| Label |
Biotin
|
| Label protocol |
Amplification and biotin-labeling were performed according to the Message Amp kit II (Ambion).
|
| |
|
| Hybridization protocol |
Codelink Expression Assay protocol
|
| Scan protocol |
Codelink Expression Assay protocol
|
| Description |
control group
|
| Data processing |
Codelink Expression analysis v4.2 software
|
| |
|
| Submission date |
Jun 12, 2008 |
| Last update date |
Jun 12, 2008 |
| Contact name |
stéphane ansieau |
| Organization name |
Inserm U590
|
| Street address |
28 rue laennec
|
| City |
Lyon |
| ZIP/Postal code |
69008 |
| Country |
France |
| |
|
| Platform ID |
GPL2897 |
| Series (1) |
| GSE11756 |
Comparison of primary MEF vs HRasV12 + Twist transformed MEF |
|
