|
| Status |
Public on Jun 10, 2009 |
| Title |
20d SM muscle of +/Cpat, biological rep 2 [RMA] |
| Sample type |
RNA |
| |
|
| Source name |
20 day old lamb semimembranosus muscle
|
| Organism |
Ovis aries |
| Characteristics |
genotype +/Cpat, age 20 days, breed 1/2 suffolk 1/2 dorset, muscle semimembranosus
|
| Treatment protocol |
Lambs were euthanized, semimembranosus muscle excised and fixed in RNAlater (Ambion).
|
| Growth protocol |
Lambs were housed indoors and allowed to nurse ewes fed grain based diet until collection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissue homogenized in 4M guanidinium thiocyanate, 25mM EDTA, 1% sodium-N-lauroyl-sarcosine. Homogenates ultracentrifuged on 5.7M CsCl cusion. RNA purified and DNase treated with Nucleospin RNA II columns (Machery-Nagel Inc.).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 5 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 micrograms of cRNA were hybridized for 16hr at 45C on GeneChip Bovine Expression Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
gene expression data using bovine platform
|
| Data processing |
Images were interpreted with Microarray Suite version 5.0 (MAS5.0) in GCOS with no scaling or normalization. Images were also interpreted by RMA using Bioconductor package of R.
The VALUEs below represent RMA signals.
|
| |
|
| Submission date |
Jun 13, 2008 |
| Last update date |
Jun 13, 2008 |
| Contact name |
Christopher A Bidwell |
| E-mail(s) |
cbidwell@purdue.edu
|
| Phone |
765-494-8016
|
| Organization name |
Purdue University
|
| Department |
Animal Sciences
|
| Street address |
125 South Russell Street
|
| City |
West Lafayette |
| State/province |
IN |
| ZIP/Postal code |
47907-2042 |
| Country |
USA |
| |
|
| Platform ID |
GPL2112 |
| Series (1) |
| GSE11780 |
Muscle gene expression of lambs with maternal callipyge allele |
|
