|
| Status |
Public on May 20, 2009 |
| Title |
In vitro matured oocytes rep1 |
| Sample type |
RNA |
| |
|
| Source name |
In vitro matured rhesus MII-stage oocytes
|
| Organism |
Macaca mulatta |
| Characteristics |
In vitro maturation
oocytes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from 4 MII-stage oocytes was extracted and purified using PicoPure kit (Arcturus).
|
| Label |
biotin
|
| Label protocol |
Total RNA from 4 MII-stage oocytes was processed through two-rounds of amplification to produce biotinylated cRNA targets using Affy Small Sample Target Labeling Assay Version II.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Gene expression data from in vitro matured MII-stage oocytes
|
| Data processing |
The data were normalized with Affymetrix Microarray Suite version 5.0 (MAS 5.0) at the mean target intensity of 150.
|
| |
|
| Submission date |
Jun 26, 2008 |
| Last update date |
May 20, 2009 |
| Contact name |
Young Shin Lee |
| Organization name |
Temple University
|
| Department |
Fels Institute for Cancer Research and Molecular Biology
|
| Street address |
3307 N. Broad Street AHB 557
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19140 |
| Country |
USA |
| |
|
| Platform ID |
GPL3535 |
| Series (1) |
| GSE11895 |
Effects of in vitro maturation on oocyte gene expression |
|
