|
| Status |
Public on Jul 06, 2009 |
| Title |
4 hrs after the third OVA challenge Lung tissue Group2_3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Placebo treated control pool (Cy3)
|
| Organism |
Mus musculus |
| Characteristics |
Balb/c mice placebo challenged control lung tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit
Quality controlled by Agilent bioanalyzer
|
| Label |
Cy3
|
| Label protocol |
Agilent dual color microarray protocol version 5.5
Data normalisation by Agilent Feature Extraction 7.5
|
| |
|
| Channel 2 |
| Source name |
OVA challenged mice
|
| Organism |
Mus musculus |
| Characteristics |
Balb/c mice treated and challenged with OVA lung tissue RNA
Group2: 4 hours after the third OVA allergen challenge (day30)
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Qiagen RNeasy kit
Quality controlled by Agilent bioanalyzer
|
| Label |
Cy5
|
| Label protocol |
Agilent dual color microarray protocol version 5.5
Data normalisation by Agilent Feature Extraction 7.5
|
| |
|
| |
| Hybridization protocol |
Agilent dual color protocol version 5.5
|
| Scan protocol |
Agilent microarray scanner default setting for 4x44k dual color microarray
|
| Description |
This is the third sample of the four biological replicates, belonging to group2
|
| Data processing |
Data were normalised by Agilent Feature Extraction 7.5
Value is normalized log10Ratio, default Per Spot and Per Chip: Intensity dependent (Lowess) normalization
|
| |
|
| Submission date |
Jun 27, 2008 |
| Last update date |
Jul 06, 2009 |
| Contact name |
Gergely Tolgyesi |
| E-mail(s) |
tolgyesi_gergely@yahoo.com
|
| Organization name |
Semmelweis University Budapest
|
| Street address |
Nagyvarad ter 4
|
| City |
Budapest |
| ZIP/Postal code |
1089 |
| Country |
Hungary |
| |
|
| Platform ID |
GPL4134 |
| Series (1) |
| GSE11911 |
Gene expression profiling of experimental asthma reveals a possible role of paraoxonase-1 in asthma |
|
