|
Status |
Public on Nov 10, 2008 |
Title |
LNCaP SOX4 hybridization 1 Replicate 2 |
Sample type |
RNA |
|
|
Source name |
LNCaP prostate cancer cells transfected with SOX4, 24 hrs
|
Organism |
Homo sapiens |
Characteristics |
LNCaP prostate cancer cells
|
Treatment protocol |
LNCaP cells were transfected with 500 ng of either a SOX4 or GFP expression vector using Lipofectamine2000 (Invitrogen) and total RNA harvested 24 hours post-transfection
|
Growth protocol |
LNCaP cells were grown in the presence of T-medium (invitrogen)
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNeasy Mini Kit
|
Label |
biotin
|
Label protocol |
standard Illumina labeling protocol
|
|
|
Hybridization protocol |
standard Illumina hybridization protocol
|
Scan protocol |
standard Illumina scanning protocol
|
Description |
Biological Replicate 2 of 3, Hybridization 1 of 2, LNCaP cells transfected with SOX4, RNA harvested 24 hrs post-transfection
|
Data processing |
Data processing performed with BeadStudio v3.1.3
|
|
|
Submission date |
Jun 27, 2008 |
Last update date |
Nov 10, 2008 |
Contact name |
Chris Scharer |
E-mail(s) |
cdschar@emory.edu
|
Organization name |
Emory University
|
Department |
Microbiology and Immunology
|
Lab |
Chris Scharer
|
Street address |
1510 Clifton Rd, Suite 3086A
|
City |
Atlanta |
State/province |
GA |
ZIP/Postal code |
30322 |
Country |
USA |
|
|
Platform ID |
GPL6102 |
Series (2) |
GSE11913 |
Illumina expression data from LNCaP cells overexpressing either SOX4 or GFP |
GSE11915 |
SOX4 effect on LNCaP prostate cancer cells |
|