|
| Status |
Public on Nov 10, 2008 |
| Title |
LNCaP GFP hybridization 2 Replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
LNCaP prostate cancer cells transfected with GFP, 24 hrs
|
| Organism |
Homo sapiens |
| Characteristics |
LNCaP prostate cancer cells
|
| Treatment protocol |
LNCaP cells were transfected with 500 ng of either a SOX4 or GFP expression vector using Lipofectamine2000 (Invitrogen) and total RNA harvested 24 hours post-transfection
|
| Growth protocol |
LNCaP cells were grown in the presence of T-medium (invitrogen)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Mini Kit
|
| Label |
biotin
|
| Label protocol |
standard Illumina labeling protocol
|
| |
|
| Hybridization protocol |
standard Illumina hybridization protocol
|
| Scan protocol |
standard Illumina scanning protocol
|
| Description |
Biological Replicate 1 of 3, Hybridization 2 of 2, LNCaP cells transfected with GFP, RNA harvested 24 hrs post-transfection
|
| Data processing |
Data processing performed with BeadStudio v3.1.3
|
| |
|
| Submission date |
Jun 27, 2008 |
| Last update date |
Nov 10, 2008 |
| Contact name |
Chris Scharer |
| E-mail(s) |
cdschar@emory.edu
|
| Organization name |
Emory University
|
| Department |
Microbiology and Immunology
|
| Lab |
Chris Scharer
|
| Street address |
1510 Clifton Rd, Suite 3086A
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platform ID |
GPL6102 |
| Series (2) |
| GSE11913 |
Illumina expression data from LNCaP cells overexpressing either SOX4 or GFP |
| GSE11915 |
SOX4 effect on LNCaP prostate cancer cells |
|
