|
| Status |
Public on Feb 14, 2019 |
| Title |
APT074 |
| Sample type |
RNA |
| |
|
| Source name |
Primary Tumor
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Primary Tumor
disease state: Wilms tumor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Midi kit. Integrity, size and quantification of total RNA was assessed using a 2100 BioAnalyzer (Agilent, Santa Clara, CA).
|
| Label |
biotin
|
| Label protocol |
Sense target cDNA was prepared from 100ng of total RNA using the Affymetrix GeneChip WT Plus (Applied Biosystems P/N 902281). cDNA was fragmented and biotin-labeled using the GeneChip(tm) WT Terminal Labeling Kit (Applied Biosystems P/N 900670).
|
| |
|
| Hybridization protocol |
Five micrograms of cDNA was hybridized for 16h at 45C on a Clariom S Human GeneChip array.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Data processing |
Probe signals were quantile normalized and summarized by the RMA algorithm using the Affymetrix Expression Console software v1.1
|
| |
|
| Submission date |
Feb 15, 2018 |
| Last update date |
Feb 14, 2019 |
| Contact name |
Tong Lin |
| Organization name |
St. Jude Children's Research Hospital
|
| Department |
Biostatistics
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL23126 |
| Series (2) |
| GSE110696 |
Gene expression data comparing Wilms tumor primary samples to patient-derived xenografts |
| GSE110699 |
Comparing Wilms tumor primary samples to patient-derived xenografts |
|
