|
| Status |
Public on Jun 21, 2020 |
| Title |
CD11b+ MDSC from spleen, biological rep6 |
| Sample type |
RNA |
| |
|
| Source name |
CD11b+ cells from spleen of mice 9 days after s.c. challenged with 5E5 C26GM tumor cells
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: BALB/c
tissue: Spleen
cell type: CD11b+ MDSC
experimental condition: Tumor-bearing
|
| Growth protocol |
Balb/c mice were challenged with 5E5 C26GM cells sc. 9 days later, CD11b+ cells were magnetically isolated (Miltenyi) from RBC lysed splenocytes or collagenase (Sigma) treated tumors. CD11b+ cells purity was higher than 95% as per flow cytometry analysis.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to manufacturer guidelines, with minor modifications. Extraction was followed by Qiagen RNeasy column clean-up according to manufacturer instructions.
|
| Label |
biotin
|
| Label protocol |
According to Expression Analysis: Technical Manual. 701021 Rev. 5. Santa Clara, CA, Affymetrix; 2004.
|
| |
|
| Hybridization protocol |
According to Expression Analysis: Technical Manual. 701021 Rev. 5. Santa Clara, CA, Affymetrix; 2004.
|
| Scan protocol |
According to Expression Analysis: Technical Manual. 701021 Rev. 5. Santa Clara, CA, Affymetrix; 2004.
|
| Description |
Cd11b_spleen_6
Gene expression profile of CD11b+ cells from spleen of mice 9 days after s.c. challenge with 5E5 C26GM tumor cells.
|
| Data processing |
RMA of .CEL files using Bioconductor affy library and Brainarray custom CDF Mouse4302_Mm_ENTREZG_21.0.0. In RMA, PM values have been background adjusted, normalized using quantile normalization, and expression measure calculated using median polish summarization.
|
| |
|
| Submission date |
Feb 18, 2018 |
| Last update date |
Jun 21, 2020 |
| Contact name |
Silvio Bicciato |
| E-mail(s) |
silvio.bicciato@unipd.it
|
| Phone |
+39-049-827-6108
|
| Organization name |
University of Padova
|
| Department |
Molecular Medicine
|
| Street address |
via U. Bassi 59/b
|
| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
| |
|
| Platform ID |
GPL23097 |
| Series (2) |
| GSE110772 |
Expression data from spleen-derived and tumor-induced CD11b+ MDSC |
| GSE110774 |
Expression data from myeloid-derived suppressor cells (MDSCs) |
|
