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Sample GSM301716 Query DataSets for GSM301716
Status Public on Jul 02, 2008
Title Adult Salivary Gland rep4
Sample type RNA
 
Source name Adult Salivary Gland
Organism Drosophila melanogaster
Characteristics tissue: Canton-S, 60 female and 60 male flies, 7 days old
Treatment protocol Flies were anaesthetised briefly by chilling on ice, then immediately dissected.
Growth protocol Wild-type Drosophila melanogaster (Canton S strain) were raised on standard medium on a 12:12 h L:D cycle, at 23C, and at 55% r.h. To facilitate the collection of accurately staged adults, a laying population of around 12 males and 12 females were transferred to fresh vials daily. When adults subsequently emerged, they were transferred to fresh vials on the day of emergence, and used 7 days later. Where larvae were used, these were third instar.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNeasy Micro kit (Invitrogen) according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
 
Hybridization protocol Following fragmentation, 10 ug of cRNA (or according to the affy IVT protocol) were hybridized for 16 hr at 45C on GeneChip Drosophila Genome 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip scanner 3000 7G.
Description The Salivary glands
amplification: 1-round
Data processing The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Jun 30, 2008
Last update date Aug 28, 2018
Contact name Jing Wang or Venkat Chintapalli
E-mail(s) jw128h@udcf.gla.ac.uk, vrc1h@udcf.gla.ac.uk
Phone 0141 3306212
Fax 3304878
Organization name University of Glasgow
Department Molecular Genetics
Lab Dow/Davis lab
Street address Dumbarton Road
City Glasgow
State/province Scotland
ZIP/Postal code G11 6NU
Country United Kingdom
 
Platform ID GPL1322
Series (1)
GSE7763 Using FlyAtlas to identify better Drosophila models of human disease
Relations
Reanalyzed by GSE119084

Data table header descriptions
ID_REF
VALUE GCOS1.4 signal intensity (MAS 5)
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 382.172 P 0.00010954
AFFX-BioB-M_at 418.375 P 5.16732e-05
AFFX-BioB-3_at 307.332 P 5.16732e-05
AFFX-BioC-5_at 818.952 P 6.02111e-05
AFFX-BioC-3_at 1114.42 P 4.42873e-05
AFFX-BioDn-5_at 2428.54 P 4.42873e-05
AFFX-BioDn-3_at 3356.08 P 4.42873e-05
AFFX-CreX-5_at 14109.2 P 5.16732e-05
AFFX-CreX-3_at 12374.7 P 4.42873e-05
AFFX-DapX-5_at 16.1773 P 0.0464816
AFFX-DapX-M_at 4.53263 A 0.382599
AFFX-DapX-3_at 1.82007 A 0.910522
AFFX-LysX-5_at 0.68774 A 0.986189
AFFX-LysX-M_at 2.51066 A 0.804734
AFFX-LysX-3_at 8.71993 A 0.156732
AFFX-PheX-5_at 1.5392 A 0.724854
AFFX-PheX-M_at 0.655102 A 0.99156
AFFX-PheX-3_at 17.0234 A 0.250796
AFFX-ThrX-5_at 0.479885 A 0.916408
AFFX-ThrX-M_at 1.62903 A 0.916408

Total number of rows: 18952

Table truncated, full table size 575 Kbytes.




Supplementary file Size Download File type/resource
GSM301716.CEL.gz 1.8 Mb (ftp)(http) CEL
GSM301716.CHP.gz 109.2 Kb (ftp)(http) CHP
GSM301716.xml.gz 4.7 Kb (ftp)(http) XML
Processed data provided as supplementary file
Processed data included within Sample table

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