|
| Status |
Public on Feb 24, 2018 |
| Title |
CF 3D post-MI, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
cardiac fibroblasts isolated from infarct area 3 days post-MI
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: TCF21-MerCreMer;R26GFP
cell type: sorted TCF21 lineage-traced cardiac fibroblasts
|
| Growth protocol |
cell isolation protocol: Heart tissue from tamoxifen-treated TCF21-MerCreMer;R26GFP mice were digested by collagenase D and Dispase II. GFP positive TCF21 cardiac fibroblasts were sorted out by FACS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol-based extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The GeneChip 3’ IVT Pico Reagent Kit is used to create biotin – labeled double -strand cDNA targets for hybridization to the Clariom S Arrays, Mouse.
|
| |
|
| Hybridization protocol |
3.0 ug of fragmented cDNA were hybridized to Clariom S Arrays, Mouse (Applied Biosystems). Probe arrays were incubated at 45°C for 17 hrs in the Hybridization Oven 640 (Affymetrix) rotating at 60 rpm. Probe arrays were washed and stained using the Fluidics Station 450 (Affymetrix), utilizing the fluidics protocol FS450-0007. The Stain and Antibody solutions are produced by Applied Biosystems and contained in the Genechip Hybridization Wash and Stain Kit.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
CF_3D MI-2
Gene expression data from cardiac fibroblasts isolated from infarct area 3 days post-MI.
|
| Data processing |
The .cel were created by Command Console (Affymetrix). The .cel files were analyzed by Expression Console (Affymetrix) to create .sst-rma-gene-full.chp files.
|
| |
|
| Submission date |
Feb 23, 2018 |
| Last update date |
Feb 24, 2018 |
| Contact name |
Vikram Prasad |
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
Molecular Cardiovascular Biology
|
| Street address |
240, Albert Sabin Way
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE111059 |
Gene expression of cardiac fibroblasts post-MI |
|
