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Sample GSM3022682 Query DataSets for GSM3022682
Status Public on Mar 06, 2018
Title arr-13-019 Blood
Sample type genomic
 
Source name Blood
Organism Homo sapiens
Characteristics Sex: Female
status: Affected
Extracted molecule genomic DNA
Extraction protocol Extraction of genomic DNA from fresh whole blood was performed using a standard salt extraction.
Label Biotin-streptavidin-green, DNP-anti-DNP-red
Label protocol 200ng of genomic DNA was whole-genome amplified in an overnight reaction at 37°C using multi-sample amplification mix 1 and 2 (MA1 and MA2) and multi-sample amplification master mix (WG-MSM). After incubation the amplified DNA was fragmented with fragmentation solution (WG-FMS), precipitated with isopropanol and precipitation solution (PM1) and resuspended in hybridization buffer (RA1).
 
Hybridization protocol RA1 resuspended DNA was loaded onto BeadChip arrays. After overnight incubation at 48°C, single-base extension and allele-specific staining was performed on a Te-Flow Flow-Through Chamber rack system (Tecan, Maennedorf, Switzerland).
Scan protocol After allele-specific staining BeadChip arrays were coated with XC4/ethanol , dried for 1 hour and scanned on a BeadArray Reader and iScan System (Illumina).
Description Genomic DNA extracted from whole blood was genotyped using Infinium Omni1-Quad Genotyping BeadChips (Illumina).
Data processing Genomic DNA extracted from whole blood was genotyped using Infinium HumanOmni1-Quad BeadChips (Illumina).
 
Submission date Feb 26, 2018
Last update date Mar 07, 2018
Contact name Barbara Vona
E-mail(s) barbara.vona@uni-wuerzburg.de
Phone +49 931-31-84244
Organization name University of Wuerzburg
Department Institute of Human Genetics
Lab Haaf Lab
Street address Biozentrum, Am Hubland
City Wuerzburg
State/province Bavaria
ZIP/Postal code 97074
Country Germany
 
Platform ID GPL24663
Series (1)
GSE111131 Hereditary Hearing Loss SNP-Microarray Pilot Study

Supplementary file Size Download File type/resource
GSM3022682_9006441135_R02C01_Grn.idat.gz 7.9 Mb (ftp)(http) IDAT
GSM3022682_9006441135_R02C01_Red.idat.gz 7.9 Mb (ftp)(http) IDAT
Processed data are available on Series record

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