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Status |
Public on Mar 01, 2018 |
Title |
Enriched astrocyte mRNA CETP_1 |
Sample type |
RNA |
|
|
Source name |
astrocyte, CETP, 1%chol. diet
|
Organism |
Mus musculus |
Characteristics |
tissue: mouse astrocytes genotype: CETP transgenic, #531 diet: 1percent cholesterol diet diet: 1% cholesterol enriched
|
Growth protocol |
CETP transgenic animals(B6.CBA-Tg(CETP)5203Tall/J) were fed for 2 months (2-4 month) with a diet containing 1% cholesterol, WT animals were fed a standard diet
|
Extracted molecule |
total RNA |
Extraction protocol |
GLAST positive astrocytes were purified from whole mouse brains using magnetic ACSA-2 affinity kit (Miltenyi biotech) according to manufacteurs instructions. Astrocyte purity was determined to be more than 70% using flow cytometry (GFAP). total RNA was extracted using the macherey & nagel RNA isolation kit according to manufacteurs instructions.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix Pico protocol from 10 ng total RNA.
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|
|
Hybridization protocol |
4 µg of ds cDNA were hybridized for 16hr at 45°C on a Mouse Clariom S array. Chip was washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
Chip was scanned using the Affymetrix GeneChip® scanner 3000
|
Data processing |
The data were analyzed with using Affymetrix default analysis settings and global scaling as normalization method. Further analysis was performed using Ingenuity pathways analysis
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Submission date |
Feb 28, 2018 |
Last update date |
Jul 14, 2022 |
Contact name |
Lisa Marie Munter |
E-mail(s) |
lisa.munter@mcgill.ca
|
Organization name |
McGill University
|
Department |
Pharmacology
|
Lab |
Munter lab
|
Street address |
3649 Prom. Sir-William-Osler
|
City |
Montreal |
State/province |
Quebec |
ZIP/Postal code |
H3G 0B1 |
Country |
Canada |
|
|
Platform ID |
GPL23038 |
Series (1) |
GSE111242 |
Astrocyte mRNA expression - comparison of wild type animals to CETP transgenic animals |
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