|
| Status |
Public on Mar 01, 2018 |
| Title |
Enriched astrocyte mRNA CETP_4 |
| Sample type |
RNA |
| |
|
| Source name |
astrocyte, CETP, std diet
|
| Organism |
Mus musculus |
| Characteristics |
tissue: mouse astrocytes
genotype: CETP transgenic, #751
diet: standard diet
diet: standard
|
| Growth protocol |
CETP transgenic animals(B6.CBA-Tg(CETP)5203Tall/J) were fed for 2 months (2-4 month) with a diet containing 1% cholesterol, WT animals were fed a standard diet
|
| Extracted molecule |
total RNA |
| Extraction protocol |
GLAST positive astrocytes were purified from whole mouse brains using magnetic ACSA-2 affinity kit (Miltenyi biotech) according to manufacteurs instructions. Astrocyte purity was determined to be more than 70% using flow cytometry (GFAP). total RNA was extracted using the macherey & nagel RNA isolation kit according to manufacteurs instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix Pico protocol from 10 ng total RNA.
|
| |
|
| Hybridization protocol |
4 µg of ds cDNA were hybridized for 16hr at 45°C on a Mouse Clariom S array. Chip was washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Chip was scanned using the Affymetrix GeneChip® scanner 3000
|
| Data processing |
The data were analyzed with using Affymetrix default analysis settings and global scaling as normalization method. Further analysis was performed using Ingenuity pathways analysis
|
| |
|
| Submission date |
Feb 28, 2018 |
| Last update date |
Jul 18, 2022 |
| Contact name |
Lisa Marie Munter |
| E-mail(s) |
lisa.munter@mcgill.ca
|
| Organization name |
McGill University
|
| Department |
Pharmacology
|
| Lab |
Munter lab
|
| Street address |
3649 Prom. Sir-William-Osler
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3G 0B1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE111242 |
Astrocyte mRNA expression - comparison of wild type animals to CETP transgenic animals |
|
