|
| Status |
Public on Mar 25, 2009 |
| Title |
transformed by GLI1 clone II, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
transformed cells by GLI1
|
| Organism |
Rattus norvegicus |
| Characteristics |
kidney cell
|
| Growth protocol |
RK3E cells were maintained in DMEM supplemented with 10%FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
400,000 RK3E cells in 60mm dish was transfected with 4 ug of GLI1 expression construct and 12 ul Lipofectamine 2000.
transformed cells form foci. The individual focus was isolated and was maintained.
total RNA was purified according to the manufacturer's instructions (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr on Rat 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner.
|
| Description |
Gene expression data from RK3E cells transformed by GLI1.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
|
| |
|
| Submission date |
Jul 03, 2008 |
| Last update date |
Mar 25, 2009 |
| Contact name |
Joon Won Yoon |
| E-mail(s) |
j-yoon2@northwestern.edu
|
| Organization name |
Children's Memorial Reseach Center
|
| Street address |
2430 N Halsted
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60614 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE11987 |
Expression data from GLI1-transformed RK3E cells |
|
