|
Status |
Public on Mar 25, 2009 |
Title |
parental RK3E, biological rep2 |
Sample type |
RNA |
|
|
Source name |
RK3E cells
|
Organism |
Rattus norvegicus |
Characteristics |
kidney cell
|
Growth protocol |
RK3E cells were maintained in DMEM supplemented with 10%FBS
|
Extracted molecule |
total RNA |
Extraction protocol |
400,000 RK3E cells in 60mm dish was transfected with 4 ug of GLI1 expression construct and 12 ul Lipofectamine 2000. transformed cells form foci. The individual focus was isolated and was maintained. total RNA was purified according to the manufacturer's instructions (Qiagen).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
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|
|
Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr on Rat 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner.
|
Description |
Gene expression data from RK3E cells.
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
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|
|
Submission date |
Jul 03, 2008 |
Last update date |
Mar 25, 2009 |
Contact name |
Joon Won Yoon |
E-mail(s) |
j-yoon2@northwestern.edu
|
Organization name |
Children's Memorial Reseach Center
|
Street address |
2430 N Halsted
|
City |
Chicago |
State/province |
IL |
ZIP/Postal code |
60614 |
Country |
USA |
|
|
Platform ID |
GPL1355 |
Series (1) |
GSE11987 |
Expression data from GLI1-transformed RK3E cells |
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