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Sample GSM303576 Query DataSets for GSM303576
Status Public on Aug 12, 2008
Title zebrafish_48hpf_control_replicate1
Sample type RNA
 
Source name zebrafish endothelial cells FACS-isolated from flk1:GFP, control
Organism Danio rerio
Characteristics Zebrafish endothelial RNA
Treatment protocol Uninjected control, 48 hpf
Extracted molecule total RNA
Extraction protocol Trizol RNA extraction
Label Biotin
Label protocol Amplified with NuGen WT-Ovation Pico amplication kit, labeled with the WT-Ovation cDNA Biotin Module V2
 
Hybridization protocol According to manufacturer's (Affymetrix) recommendations
Scan protocol According to manufacturer's (Affymetrix) recommendations
Description zebrafish endothelial cells FACS-isolated from flk1:GFP, control
Data processing Raw intensities from CEL files were analyzed using Affymetrix Power Tool (APT, version 1.8.5) to generate robust multi-array average intensity in log2 scale for each probe set. The perfect match intensities for each probe set were corrected for background and quantile-normalized using a robust fit of linear models.
 
Submission date Jul 04, 2008
Last update date Aug 12, 2008
Contact name Jason E. Fish
E-mail(s) jefish@gladstone.ucsf.edu
Organization name J. David Gladstone Institute
Department Gladstone Institute of Cardiovascular Disease
Lab Deepak Srivastava
Street address 1650 Owens Street
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL1319
Series (2)
GSE12012 Regulation of zebrafish endothelial gene expression by miR-126
GSE12039 Regulation of endothelial gene expression by miR-126 in human and zebrafish

Data table header descriptions
ID_REF
VALUE normalized RMA signal (log2 scale)

Data table
ID_REF VALUE
AFFX-BioB-3_at 10.36568878
AFFX-BioB-5_at 9.77775968
AFFX-BioB-M_at 10.46997078
AFFX-BioC-3_at 9.77247993
AFFX-BioC-5_at 10.8243698
AFFX-BioDn-3_at 12.85590292
AFFX-BioDn-5_at 11.00138043
AFFX-CreX-3_at 14.19163938
AFFX-CreX-5_at 13.50571253
AFFX-DapX-3_at 4.25622356
AFFX-DapX-5_at 5.074745413
AFFX-DapX-M_at 3.938758015
AFFX-Dr-AB076373-1_at 5.357653561
AFFX-Dr-AF292559-1_at 2.992980981
AFFX-Dr-AF292559-2_s_at 3.175910979
AFFX-Dr-AF292559-3_s_at 3.005281469
AFFX-Dr-AF292559-4_s_at 4.104499593
AFFX-Dr-AF292560-1_s_at 3.185296577
AFFX-Dr-AF298789-1_at 5.173617215
AFFX-Dr-AF323980-1_at 3.611513168

Total number of rows: 15617

Table truncated, full table size 436 Kbytes.




Supplementary file Size Download File type/resource
GSM303576.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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