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Sample GSM303577 Query DataSets for GSM303577
Status Public on Aug 12, 2008
Title zebrafish_48hpf_miR-126 MO-1_replicate1
Sample type RNA
 
Source name zebrafish endothelial cells FACS-isolated from flk1:GFP, miR-126 MO#1
Organism Danio rerio
Characteristics zebrafish endothelial cell RNA
Treatment protocol zebrafish embryos were injected at the one-cell stae with miR-126 antisense morpholino (4 ng, Gene-Tools), RNA isolated from 48 hpf fish
Extracted molecule total RNA
Extraction protocol Trizol RNA extraction
Label Biotin
Label protocol Amplified with NuGen WT-Ovation Pico amplication kit, labeled with the WT-Ovation cDNA Biotin Module V2
 
Hybridization protocol According to manufacturer's (Affymetrix) recommendations
Scan protocol According to manufacturer's (Affymetrix) recommendations
Description zebrafish endothelial cells FACS-isolated from flk1:GFP, miR-126 MO#1
Data processing Raw intensities from CEL files were analyzed using Affymetrix Power Tool (APT, version 1.8.5) to generate robust multi-array average intensity in log2 scale for each probe set. The perfect match intensities for each probe set were corrected for background and quantile-normalized using a robust fit of linear models.
 
Submission date Jul 04, 2008
Last update date Aug 12, 2008
Contact name Jason E. Fish
E-mail(s) jefish@gladstone.ucsf.edu
Organization name J. David Gladstone Institute
Department Gladstone Institute of Cardiovascular Disease
Lab Deepak Srivastava
Street address 1650 Owens Street
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL1319
Series (2)
GSE12012 Regulation of zebrafish endothelial gene expression by miR-126
GSE12039 Regulation of endothelial gene expression by miR-126 in human and zebrafish

Data table header descriptions
ID_REF
VALUE normalized RMA signal (log2 scale)

Data table
ID_REF VALUE
AFFX-BioB-3_at 10.39999138
AFFX-BioB-5_at 9.692270649
AFFX-BioB-M_at 10.44969289
AFFX-BioC-3_at 9.773272224
AFFX-BioC-5_at 10.78804843
AFFX-BioDn-3_at 12.87251265
AFFX-BioDn-5_at 11.04505882
AFFX-CreX-3_at 14.22791108
AFFX-CreX-5_at 13.47861517
AFFX-DapX-3_at 4.27088768
AFFX-DapX-5_at 5.08275023
AFFX-DapX-M_at 3.840387819
AFFX-Dr-AB076373-1_at 5.45622123
AFFX-Dr-AF292559-1_at 3.155765807
AFFX-Dr-AF292559-2_s_at 3.030459514
AFFX-Dr-AF292559-3_s_at 2.894739813
AFFX-Dr-AF292559-4_s_at 4.090588385
AFFX-Dr-AF292560-1_s_at 3.244361625
AFFX-Dr-AF298789-1_at 5.094915256
AFFX-Dr-AF323980-1_at 3.557341545

Total number of rows: 15617

Table truncated, full table size 436 Kbytes.




Supplementary file Size Download File type/resource
GSM303577.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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