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Sample GSM303579 Query DataSets for GSM303579
Status Public on Aug 12, 2008
Title zebrafish_48hpf_control_replicate2
Sample type RNA
 
Source name zebrafish endothelial cells FACS-isolated from flk1:GFP, control
Organism Danio rerio
Characteristics zebrafish endothelial cell RNA
Treatment protocol RNA isolated from 48 hpf fish
Extracted molecule total RNA
Extraction protocol Trizol RNA extraction
Label Biotin
Label protocol Amplified with NuGen WT-Ovation Pico amplication kit, labeled with the WT-Ovation cDNA Biotin Module V2
 
Hybridization protocol According to manufacturer's (Affymetrix) recommendations
Scan protocol According to manufacturer's (Affymetrix) recommendations
Description zebrafish endothelial cells FACS-isolated from flk1:GFP, control
Data processing Raw intensities from CEL files were analyzed using Affymetrix Power Tool (APT, version 1.8.5) to generate robust multi-array average intensity in log2 scale for each probe set. The perfect match intensities for each probe set were corrected for background and quantile-normalized using a robust fit of linear models.
 
Submission date Jul 04, 2008
Last update date Aug 12, 2008
Contact name Jason E. Fish
E-mail(s) jefish@gladstone.ucsf.edu
Organization name J. David Gladstone Institute
Department Gladstone Institute of Cardiovascular Disease
Lab Deepak Srivastava
Street address 1650 Owens Street
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL1319
Series (2)
GSE12012 Regulation of zebrafish endothelial gene expression by miR-126
GSE12039 Regulation of endothelial gene expression by miR-126 in human and zebrafish

Data table header descriptions
ID_REF
VALUE normalized RMA signal (log2 scale)

Data table
ID_REF VALUE
AFFX-BioB-3_at 10.43564053
AFFX-BioB-5_at 9.827963477
AFFX-BioB-M_at 10.46008944
AFFX-BioC-3_at 9.837393454
AFFX-BioC-5_at 10.88768023
AFFX-BioDn-3_at 12.87755901
AFFX-BioDn-5_at 11.00818121
AFFX-CreX-3_at 14.16062428
AFFX-CreX-5_at 13.43456958
AFFX-DapX-3_at 4.362762863
AFFX-DapX-5_at 5.169355049
AFFX-DapX-M_at 3.992356239
AFFX-Dr-AB076373-1_at 5.327270103
AFFX-Dr-AF292559-1_at 3.027953095
AFFX-Dr-AF292559-2_s_at 3.136672826
AFFX-Dr-AF292559-3_s_at 3.108981192
AFFX-Dr-AF292559-4_s_at 3.868903589
AFFX-Dr-AF292560-1_s_at 3.331763148
AFFX-Dr-AF298789-1_at 5.152382308
AFFX-Dr-AF323980-1_at 3.442973808

Total number of rows: 15617

Table truncated, full table size 436 Kbytes.




Supplementary file Size Download File type/resource
GSM303579.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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