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Sample GSM303580 Query DataSets for GSM303580
Status Public on Aug 12, 2008
Title zebrafish_48hpf_miR-126 MO-1_replicate2
Sample type RNA
 
Source name zebrafish endothelial cells FACS-isolated from flk1:GFP, miR-126 MO#1
Organism Danio rerio
Characteristics zebrafish endothelial cell RNA
Treatment protocol zebrafish embryos at the one cell stage were injected with miR-126 antisense morpholino (4 ng, Gene Tools), RNA isolated from 48 hpf fish
Extracted molecule total RNA
Extraction protocol Trizol RNA extraction
Label Biotin
Label protocol Amplified with NuGen WT-Ovation Pico amplication kit, labeled with the WT-Ovation cDNA Biotin Module V2
 
Hybridization protocol According to manufacturer's (Affymetrix) recommendations
Scan protocol According to manufacturer's (Affymetrix) recommendations
Description zebrafish endothelial cells FACS-isolated from flk1:GFP, miR-126 MO#1
Data processing Raw intensities from CEL files were analyzed using Affymetrix Power Tool (APT, version 1.8.5) to generate robust multi-array average intensity in log2 scale for each probe set. The perfect match intensities for each probe set were corrected for background and quantile-normalized using a robust fit of linear models.
 
Submission date Jul 04, 2008
Last update date Aug 12, 2008
Contact name Jason E. Fish
E-mail(s) jefish@gladstone.ucsf.edu
Organization name J. David Gladstone Institute
Department Gladstone Institute of Cardiovascular Disease
Lab Deepak Srivastava
Street address 1650 Owens Street
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL1319
Series (2)
GSE12012 Regulation of zebrafish endothelial gene expression by miR-126
GSE12039 Regulation of endothelial gene expression by miR-126 in human and zebrafish

Data table header descriptions
ID_REF
VALUE normalized RMA signal (log2 scale)

Data table
ID_REF VALUE
AFFX-BioB-3_at 10.54391659
AFFX-BioB-5_at 9.83286383
AFFX-BioB-M_at 10.54138373
AFFX-BioC-3_at 9.968096154
AFFX-BioC-5_at 10.94470787
AFFX-BioDn-3_at 12.88062629
AFFX-BioDn-5_at 11.09307133
AFFX-CreX-3_at 14.30723277
AFFX-CreX-5_at 13.55289248
AFFX-DapX-3_at 4.085576954
AFFX-DapX-5_at 4.972281946
AFFX-DapX-M_at 3.801376355
AFFX-Dr-AB076373-1_at 4.991481037
AFFX-Dr-AF292559-1_at 3.161027898
AFFX-Dr-AF292559-2_s_at 2.998434402
AFFX-Dr-AF292559-3_s_at 3.031349114
AFFX-Dr-AF292559-4_s_at 3.697607267
AFFX-Dr-AF292560-1_s_at 3.199875016
AFFX-Dr-AF298789-1_at 5.094362674
AFFX-Dr-AF323980-1_at 3.28564645

Total number of rows: 15617

Table truncated, full table size 436 Kbytes.




Supplementary file Size Download File type/resource
GSM303580.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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