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Sample GSM303581 Query DataSets for GSM303581
Status Public on Aug 12, 2008
Title zebrafish_48hpf_miR-126 MO-2_replicate2
Sample type RNA
Source name zebrafish endothelial cells FACS-isolated from flk1:GFP, miR-126 MO#2
Organism Danio rerio
Characteristics zebrafish endothelial cell RNA
Treatment protocol zebrafish embryos at the one cell stage were injected with miR-126 antisense morpholino (4 ng, Gene Tools), RNA isolated from 48 hpf fish
Extracted molecule total RNA
Extraction protocol Trizol RNA extraction
Label Biotin
Label protocol Amplified with NuGen WT-Ovation Pico amplication kit, labeled with the WT-Ovation cDNA Biotin Module V2
Hybridization protocol According to manufacturer's (Affymetrix) recommendations
Scan protocol According to manufacturer's (Affymetrix) recommendations
Description zebrafish endothelial cells FACS-isolated from flk1:GFP, miR-126 MO#2
Data processing Raw intensities from CEL files were analyzed using Affymetrix Power Tool (APT, version 1.8.5) to generate robust multi-array average intensity in log2 scale for each probe set. The perfect match intensities for each probe set were corrected for background and quantile-normalized using a robust fit of linear models.
Submission date Jul 04, 2008
Last update date Aug 12, 2008
Contact name Jason E. Fish
Organization name J. David Gladstone Institute
Department Gladstone Institute of Cardiovascular Disease
Lab Deepak Srivastava
Street address 1650 Owens Street
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
Platform ID GPL1319
Series (2)
GSE12012 Regulation of zebrafish endothelial gene expression by miR-126
GSE12039 Regulation of endothelial gene expression by miR-126 in human and zebrafish

Data table header descriptions
VALUE normalized RMA signal (log2 scale)

Data table
AFFX-BioB-3_at 10.50579485
AFFX-BioB-5_at 9.93794173
AFFX-BioB-M_at 10.73820293
AFFX-BioC-3_at 9.946275642
AFFX-BioC-5_at 10.97136728
AFFX-BioDn-3_at 12.87055804
AFFX-BioDn-5_at 11.0339851
AFFX-CreX-3_at 14.02964824
AFFX-CreX-5_at 13.4210589
AFFX-DapX-3_at 4.143860222
AFFX-DapX-5_at 5.215567871
AFFX-DapX-M_at 3.976610426
AFFX-Dr-AB076373-1_at 5.353862679
AFFX-Dr-AF292559-1_at 3.026535831
AFFX-Dr-AF292559-2_s_at 3.340908414
AFFX-Dr-AF292559-3_s_at 2.949695675
AFFX-Dr-AF292559-4_s_at 3.938403896
AFFX-Dr-AF292560-1_s_at 3.293065068
AFFX-Dr-AF298789-1_at 5.26197557
AFFX-Dr-AF323980-1_at 3.524716789

Total number of rows: 15617

Table truncated, full table size 436 Kbytes.

Supplementary file Size Download File type/resource
GSM303581.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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