|
| Status |
Public on Aug 12, 2008 |
| Title |
zebrafish_48hpf_miR-126 MO-2_replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
zebrafish endothelial cells FACS-isolated from flk1:GFP, miR-126 MO#2
|
| Organism |
Danio rerio |
| Characteristics |
zebrafish endothelial cell RNA
|
| Treatment protocol |
zebrafish embryos at the one cell stage were injected with miR-126 antisense morpholino (4 ng, Gene Tools), RNA isolated from 48 hpf fish
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol RNA extraction
|
| Label |
Biotin
|
| Label protocol |
Amplified with NuGen WT-Ovation Pico amplication kit, labeled with the WT-Ovation cDNA Biotin Module V2
|
| |
|
| Hybridization protocol |
According to manufacturer's (Affymetrix) recommendations
|
| Scan protocol |
According to manufacturer's (Affymetrix) recommendations
|
| Description |
zebrafish endothelial cells FACS-isolated from flk1:GFP, miR-126 MO#2
|
| Data processing |
Raw intensities from CEL files were analyzed using Affymetrix Power Tool (APT, version 1.8.5) to generate robust multi-array average intensity in log2 scale for each probe set. The perfect match intensities for each probe set were corrected for background and quantile-normalized using a robust fit of linear models.
|
| |
|
| Submission date |
Jul 04, 2008 |
| Last update date |
Aug 12, 2008 |
| Contact name |
Jason E. Fish |
| E-mail(s) |
jefish@gladstone.ucsf.edu
|
| Organization name |
J. David Gladstone Institute
|
| Department |
Gladstone Institute of Cardiovascular Disease
|
| Lab |
Deepak Srivastava
|
| Street address |
1650 Owens Street
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL1319 |
| Series (2) |
| GSE12012 |
Regulation of zebrafish endothelial gene expression by miR-126 |
| GSE12039 |
Regulation of endothelial gene expression by miR-126 in human and zebrafish |
|
