NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM303581 Query DataSets for GSM303581
Status Public on Aug 12, 2008
Title zebrafish_48hpf_miR-126 MO-2_replicate2
Sample type RNA
 
Source name zebrafish endothelial cells FACS-isolated from flk1:GFP, miR-126 MO#2
Organism Danio rerio
Characteristics zebrafish endothelial cell RNA
Treatment protocol zebrafish embryos at the one cell stage were injected with miR-126 antisense morpholino (4 ng, Gene Tools), RNA isolated from 48 hpf fish
Extracted molecule total RNA
Extraction protocol Trizol RNA extraction
Label Biotin
Label protocol Amplified with NuGen WT-Ovation Pico amplication kit, labeled with the WT-Ovation cDNA Biotin Module V2
 
Hybridization protocol According to manufacturer's (Affymetrix) recommendations
Scan protocol According to manufacturer's (Affymetrix) recommendations
Description zebrafish endothelial cells FACS-isolated from flk1:GFP, miR-126 MO#2
Data processing Raw intensities from CEL files were analyzed using Affymetrix Power Tool (APT, version 1.8.5) to generate robust multi-array average intensity in log2 scale for each probe set. The perfect match intensities for each probe set were corrected for background and quantile-normalized using a robust fit of linear models.
 
Submission date Jul 04, 2008
Last update date Aug 12, 2008
Contact name Jason E. Fish
E-mail(s) jefish@gladstone.ucsf.edu
Organization name J. David Gladstone Institute
Department Gladstone Institute of Cardiovascular Disease
Lab Deepak Srivastava
Street address 1650 Owens Street
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL1319
Series (2)
GSE12012 Regulation of zebrafish endothelial gene expression by miR-126
GSE12039 Regulation of endothelial gene expression by miR-126 in human and zebrafish

Data table header descriptions
ID_REF
VALUE normalized RMA signal (log2 scale)

Data table
ID_REF VALUE
AFFX-BioB-3_at 10.50579485
AFFX-BioB-5_at 9.93794173
AFFX-BioB-M_at 10.73820293
AFFX-BioC-3_at 9.946275642
AFFX-BioC-5_at 10.97136728
AFFX-BioDn-3_at 12.87055804
AFFX-BioDn-5_at 11.0339851
AFFX-CreX-3_at 14.02964824
AFFX-CreX-5_at 13.4210589
AFFX-DapX-3_at 4.143860222
AFFX-DapX-5_at 5.215567871
AFFX-DapX-M_at 3.976610426
AFFX-Dr-AB076373-1_at 5.353862679
AFFX-Dr-AF292559-1_at 3.026535831
AFFX-Dr-AF292559-2_s_at 3.340908414
AFFX-Dr-AF292559-3_s_at 2.949695675
AFFX-Dr-AF292559-4_s_at 3.938403896
AFFX-Dr-AF292560-1_s_at 3.293065068
AFFX-Dr-AF298789-1_at 5.26197557
AFFX-Dr-AF323980-1_at 3.524716789

Total number of rows: 15617

Table truncated, full table size 436 Kbytes.




Supplementary file Size Download File type/resource
GSM303581.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap