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Sample GSM303582 Query DataSets for GSM303582
Status Public on Aug 12, 2008
Title zebrafish_48hpf_control_replicate3
Sample type RNA
 
Source name zebrafish endothelial cells FACS-isolated from flk1:GFP, control
Organism Danio rerio
Characteristics zebrafish endothelial cell RNA
Treatment protocol RNA isolated from 48 hpf fish
Extracted molecule total RNA
Extraction protocol Trizol RNA extraction
Label Biotin
Label protocol Amplified with NuGen WT-Ovation Pico amplication kit, labeled with the WT-Ovation cDNA Biotin Module V2
 
Hybridization protocol According to manufacturer's (Affymetrix) recommendations
Scan protocol According to manufacturer's (Affymetrix) recommendations
Description zebrafish endothelial cells FACS-isolated from flk1:GFP, control
Data processing Raw intensities from CEL files were analyzed using Affymetrix Power Tool (APT, version 1.8.5) to generate robust multi-array average intensity in log2 scale for each probe set. The perfect match intensities for each probe set were corrected for background and quantile-normalized using a robust fit of linear models.
 
Submission date Jul 04, 2008
Last update date Aug 12, 2008
Contact name Jason E. Fish
E-mail(s) jefish@gladstone.ucsf.edu
Organization name J. David Gladstone Institute
Department Gladstone Institute of Cardiovascular Disease
Lab Deepak Srivastava
Street address 1650 Owens Street
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL1319
Series (2)
GSE12012 Regulation of zebrafish endothelial gene expression by miR-126
GSE12039 Regulation of endothelial gene expression by miR-126 in human and zebrafish

Data table header descriptions
ID_REF
VALUE normalized RMA signal (log2 scale)

Data table
ID_REF VALUE
AFFX-BioB-3_at 10.01724717
AFFX-BioB-5_at 9.479981402
AFFX-BioB-M_at 10.15259142
AFFX-BioC-3_at 9.454343912
AFFX-BioC-5_at 10.49389437
AFFX-BioDn-3_at 12.6432109
AFFX-BioDn-5_at 10.6444479
AFFX-CreX-3_at 13.99444634
AFFX-CreX-5_at 13.27528131
AFFX-DapX-3_at 4.196396461
AFFX-DapX-5_at 4.901801451
AFFX-DapX-M_at 3.814265747
AFFX-Dr-AB076373-1_at 5.395571927
AFFX-Dr-AF292559-1_at 3.147105331
AFFX-Dr-AF292559-2_s_at 3.160346902
AFFX-Dr-AF292559-3_s_at 2.87213582
AFFX-Dr-AF292559-4_s_at 3.762441805
AFFX-Dr-AF292560-1_s_at 3.453231791
AFFX-Dr-AF298789-1_at 5.038948508
AFFX-Dr-AF323980-1_at 3.511642017

Total number of rows: 15617

Table truncated, full table size 436 Kbytes.




Supplementary file Size Download File type/resource
GSM303582.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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