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Sample GSM303585 Query DataSets for GSM303585
Status Public on Aug 12, 2008
Title zebrafish_48hpf_control_replicate4
Sample type RNA
 
Source name zebrafish endothelial cells FACS-isolated from flk1:GFP, control
Organism Danio rerio
Characteristics zebrafish endothelial cell RNA
Treatment protocol RNA isolated from 48 hpf fish
Extracted molecule total RNA
Extraction protocol Trizol RNA extraction
Label Biotin
Label protocol Amplified with NuGen WT-Ovation Pico amplication kit, labeled with the WT-Ovation cDNA Biotin Module V2
 
Hybridization protocol According to manufacturer's (Affymetrix) recommendations
Scan protocol According to manufacturer's (Affymetrix) recommendations
Description zebrafish endothelial cells FACS-isolated from flk1:GFP, control
Data processing Raw intensities from CEL files were analyzed using Affymetrix Power Tool (APT, version 1.8.5) to generate robust multi-array average intensity in log2 scale for each probe set. The perfect match intensities for each probe set were corrected for background and quantile-normalized using a robust fit of linear models.
 
Submission date Jul 04, 2008
Last update date Aug 12, 2008
Contact name Jason E. Fish
E-mail(s) jefish@gladstone.ucsf.edu
Organization name J. David Gladstone Institute
Department Gladstone Institute of Cardiovascular Disease
Lab Deepak Srivastava
Street address 1650 Owens Street
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL1319
Series (2)
GSE12012 Regulation of zebrafish endothelial gene expression by miR-126
GSE12039 Regulation of endothelial gene expression by miR-126 in human and zebrafish

Data table header descriptions
ID_REF
VALUE normalized RMA signal (log2 scale)

Data table
ID_REF VALUE
AFFX-BioB-3_at 10.19808809
AFFX-BioB-5_at 9.479943162
AFFX-BioB-M_at 10.2660323
AFFX-BioC-3_at 9.653642208
AFFX-BioC-5_at 10.66936462
AFFX-BioDn-3_at 12.74558195
AFFX-BioDn-5_at 10.78220441
AFFX-CreX-3_at 14.1649503
AFFX-CreX-5_at 13.35690522
AFFX-DapX-3_at 4.138559749
AFFX-DapX-5_at 5.051171371
AFFX-DapX-M_at 3.966625247
AFFX-Dr-AB076373-1_at 5.349454708
AFFX-Dr-AF292559-1_at 3.464035527
AFFX-Dr-AF292559-2_s_at 3.130267721
AFFX-Dr-AF292559-3_s_at 2.841026748
AFFX-Dr-AF292559-4_s_at 3.902308041
AFFX-Dr-AF292560-1_s_at 3.381931958
AFFX-Dr-AF298789-1_at 5.235681721
AFFX-Dr-AF323980-1_at 3.58306976

Total number of rows: 15617

Table truncated, full table size 436 Kbytes.




Supplementary file Size Download File type/resource
GSM303585.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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