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Sample GSM303587 Query DataSets for GSM303587
Status Public on Aug 12, 2008
Title zebrafish_48hpf_miR-126 MO-2_replicate4
Sample type RNA
 
Source name zebrafish endothelial cells FACS-isolated from flk1:GFP, miR-126 MO#2
Organism Danio rerio
Characteristics zebrafish endothelial cell RNA
Treatment protocol zebrafish embryos at the one cell stage were injected with miR-126 antisense morpholino (4 ng, Gene Tools), RNA isolated from 48 hpf fish
Extracted molecule total RNA
Extraction protocol Trizol RNA extraction
Label Biotin
Label protocol Amplified with NuGen WT-Ovation Pico amplication kit, labeled with the WT-Ovation cDNA Biotin Module V2
 
Hybridization protocol According to manufacturer's (Affymetrix) recommendations
Scan protocol According to manufacturer's (Affymetrix) recommendations
Description zebrafish endothelial cells FACS-isolated from flk1:GFP, miR-126 MO#2
Data processing Raw intensities from CEL files were analyzed using Affymetrix Power Tool (APT, version 1.8.5) to generate robust multi-array average intensity in log2 scale for each probe set. The perfect match intensities for each probe set were corrected for background and quantile-normalized using a robust fit of linear models.
 
Submission date Jul 04, 2008
Last update date Aug 12, 2008
Contact name Jason E. Fish
E-mail(s) jefish@gladstone.ucsf.edu
Organization name J. David Gladstone Institute
Department Gladstone Institute of Cardiovascular Disease
Lab Deepak Srivastava
Street address 1650 Owens Street
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL1319
Series (2)
GSE12012 Regulation of zebrafish endothelial gene expression by miR-126
GSE12039 Regulation of endothelial gene expression by miR-126 in human and zebrafish

Data table header descriptions
ID_REF
VALUE normalized RMA signal (log2 scale)

Data table
ID_REF VALUE
AFFX-BioB-3_at 10.48449889
AFFX-BioB-5_at 9.936825563
AFFX-BioB-M_at 10.56307811
AFFX-BioC-3_at 9.872179675
AFFX-BioC-5_at 10.89668561
AFFX-BioDn-3_at 12.97110918
AFFX-BioDn-5_at 11.09299991
AFFX-CreX-3_at 14.22245
AFFX-CreX-5_at 13.65340624
AFFX-DapX-3_at 4.184399494
AFFX-DapX-5_at 4.869874942
AFFX-DapX-M_at 3.843690745
AFFX-Dr-AB076373-1_at 4.92749162
AFFX-Dr-AF292559-1_at 3.438464168
AFFX-Dr-AF292559-2_s_at 3.128319124
AFFX-Dr-AF292559-3_s_at 2.782657472
AFFX-Dr-AF292559-4_s_at 3.71089034
AFFX-Dr-AF292560-1_s_at 3.228075012
AFFX-Dr-AF298789-1_at 4.989462147
AFFX-Dr-AF323980-1_at 3.472644576

Total number of rows: 15617

Table truncated, full table size 436 Kbytes.




Supplementary file Size Download File type/resource
GSM303587.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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