GEO help: Mouse over screen elements for information.
Status
Public on Jul 08, 2008
Title
Induced beta cells 3
Sample type
RNA
Source name
induced beta cells
Organism
Mus musculus Characteristics
adult mouse GFP+
Extracted molecule
total RNA
Extraction protocol
~5000 GFP+ cells, 4-5 islets, or ~10,000 no islet cells per sample was extracted for total RNA with trizol reagent (invitorgen)
Label
biotin
Label protocol
Total prep RNA amplification kit (Ambion)
Hybridization protocol
standard as recommended by illumina
Scan protocol
standard as recommended by illumina
Description
rag-/- adult pancreas infected with M3 adeno virus 1 month FACS sorted contain 22% ins+ cells
Data processing
with Beadstudio, standardized. Differentially expressed genes identified with illumina recommended parameters: pvalue<0.05, diff score >30, average signal >100
Submission date
Jul 07, 2008
Last update date
Jul 07, 2008
Contact name
qiao zhou
E-mail(s)
qiao@mcb.harvard.edu Phone
617 495 2918
Organization name
Harvard University
Department
Stem cell and regenerative biology
Street address
7 Divinity Ave
City
Cambridge
State/province
MA
ZIP/Postal code
02143
Country
USA
Platform ID
GPL6103 Series (1)
GSE12025 Comparison of endocrine enriched genes in islet beta cells versus induced beta cells
Data table header descriptions ID_REF VALUE normalized signal intensity (average normalization)
Avg_NBEADS BEAD_STDERR Detection Pval
Data table ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval ILMN_1238136
2.478652
42
0.6538436
0.08953342
ILMN_2721178
72.48961
40
3.374722
0
ILMN_2773981
98.86498
43
4.738434
0
ILMN_2771349
72.81995
50
3.90815
0
ILMN_2634566
72.27399
45
3.387104
0
ILMN_2737647
0.8565464
37
0.9035471
0.2774275
ILMN_2744574
0.411402
45
0.5111812
0.372005
ILMN_2739275
1.5952
36
0.5447078
0.1689786
ILMN_2734484
74.74084
59
2.256526
0
ILMN_2699078
-1.782173
34
0.6046041
0.8713745
ILMN_2692376
6.85626
41
1.118718
0
ILMN_1252243
127.7173
41
4.678868
0
ILMN_1213681
42.07951
38
2.249837
0
ILMN_2735415
0.6179789
26
0.7808963
0.3139975
ILMN_1237980
10.63241
25
1.467898
0
ILMN_3162350
5.802029
55
0.8219239
0
ILMN_2712923
9.729517
48
1.032122
0
ILMN_2670314
25.58803
51
1.456484
0
ILMN_2637698
214.6266
23
8.531584
0
ILMN_2674228
40.93975
26
4.95807
0
24613 986 Kbytes .Supplementary data files not provided Processed data included within Sample table
