GEO help: Mouse over screen elements for information.
Status
Public on Jul 08, 2008
Title
Whole islet 1
Sample type
RNA
Source name
whole islet
Organism
Mus musculus Characteristics
adult mouse pdx1-GFP
Extracted molecule
total RNA
Extraction protocol
~5000 GFP+ cells, 4-5 islets, or ~10,000 no islet cells per sample was extracted for total RNA with trizol reagent (invitorgen)
Label
biotin
Label protocol
Total prep RNA amplification kit (Ambion)
Hybridization protocol
standard as recommended by illumina
Scan protocol
standard as recommended by illumina
Description
iselts hand picked from adult pdx-gfp transgenic animals
Data processing
with Beadstudio, standardized. Differentially expressed genes identified with illumina recommended parameters: pvalue<0.05, diff score >30, average signal >100
Submission date
Jul 07, 2008
Last update date
Jul 07, 2008
Contact name
qiao zhou
E-mail(s)
qiao@mcb.harvard.edu Phone
617 495 2918
Organization name
Harvard University
Department
Stem cell and regenerative biology
Street address
7 Divinity Ave
City
Cambridge
State/province
MA
ZIP/Postal code
02143
Country
USA
Platform ID
GPL6103 Series (1)
GSE12025 Comparison of endocrine enriched genes in islet beta cells versus induced beta cells
Data table header descriptions ID_REF VALUE normalized signal intensity (average normalization)
Avg_NBEADS BEAD_STDERR Detection Pval
Data table ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval ILMN_1238136
0.5153815
30
1.046928
0.3545918
ILMN_2721178
105.4471
33
5.041505
0
ILMN_2773981
69.48382
42
3.174995
0
ILMN_2771349
102.0245
50
4.875229
0
ILMN_2634566
94.63631
50
4.668527
0
ILMN_2737647
3.469022
43
0.9387978
0.08290816
ILMN_2744574
0.3899153
34
1.062673
0.369898
ILMN_2739275
0.6261173
41
0.9989878
0.3392857
ILMN_2734484
99.08475
43
4.373164
0
ILMN_2699078
-2.520794
37
0.860445
0.8915817
ILMN_2692376
7.153014
34
1.102843
0
ILMN_1252243
146.0362
39
5.56427
0
ILMN_1213681
75.45818
31
4.994555
0
ILMN_2735415
0.9087197
46
1.181088
0.3048469
ILMN_1237980
1.238696
43
0.933617
0.2563775
ILMN_3162350
7.760888
57
0.8307
0
ILMN_2712923
16.36435
40
1.659317
0
ILMN_2670314
46.43753
44
2.478744
0
ILMN_2637698
218.2828
39
8.812895
0
ILMN_2674228
27.2453
30
2.979812
0
24613 983 Kbytes .Supplementary data files not provided Processed data included within Sample table
