GEO help: Mouse over screen elements for information.
Status
Public on Jul 08, 2008
Title
Whole islet 2
Sample type
RNA
Source name
whole islet
Organism
Mus musculus Characteristics
adult mouse pdx1-GFP
Extracted molecule
total RNA
Extraction protocol
~5000 GFP+ cells, 4-5 islets, or ~10,000 no islet cells per sample was extracted for total RNA with trizol reagent (invitorgen)
Label
biotin
Label protocol
Total prep RNA amplification kit (Ambion)
Hybridization protocol
standard as recommended by illumina
Scan protocol
standard as recommended by illumina
Description
iselts hand picked from adult pdx-gfp transgenic animals
Data processing
with Beadstudio, standardized. Differentially expressed genes identified with illumina recommended parameters: pvalue<0.05, diff score >30, average signal >100
Submission date
Jul 07, 2008
Last update date
Jul 07, 2008
Contact name
qiao zhou
E-mail(s)
qiao@mcb.harvard.edu Phone
617 495 2918
Organization name
Harvard University
Department
Stem cell and regenerative biology
Street address
7 Divinity Ave
City
Cambridge
State/province
MA
ZIP/Postal code
02143
Country
USA
Platform ID
GPL6103 Series (1)
GSE12025 Comparison of endocrine enriched genes in islet beta cells versus induced beta cells
Data table header descriptions ID_REF VALUE normalized signal intensity (average normalization)
Avg_NBEADS BEAD_STDERR Detection Pval
Data table ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval ILMN_1238136
1.634261
34
0.9466197
0.2040816
ILMN_2721178
90.1184
43
4.041124
0
ILMN_2773981
68.22082
36
3.526178
0
ILMN_2771349
102.5715
46
4.883466
0
ILMN_2634566
100.772
40
5.260605
0
ILMN_2737647
1.504187
42
0.7347637
0.2244898
ILMN_2744574
0.1836527
33
0.899492
0.4017857
ILMN_2739275
0.4017772
41
0.8431875
0.3660714
ILMN_2734484
92.44899
47
4.521246
0
ILMN_2699078
-3.550436
34
0.6630077
0.9846939
ILMN_2692376
13.7771
43
1.741067
0
ILMN_1252243
167.0313
33
5.671287
0
ILMN_1213681
57.73383
44
3.333414
0
ILMN_2735415
-0.0866425
36
0.7475937
0.4668367
ILMN_1237980
-1.70447
30
0.973933
0.7895408
ILMN_3162350
5.970124
46
0.6750752
0.00255102
ILMN_2712923
19.21356
45
1.396909
0
ILMN_2670314
52.40503
42
2.292248
0
ILMN_2637698
293.9118
35
11.39324
0
ILMN_2674228
28.29168
35
2.395668
0
24613 984 Kbytes .Supplementary data files not provided Processed data included within Sample table
