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Status |
Public on Dec 04, 2008 |
Title |
Kidney_wild type mouse_rep1 |
Sample type |
RNA |
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Source name |
Kidney from wild type mouse
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Organism |
Mus musculus |
Characteristics |
Kidney isolated from wild type and Lmx1b conventional knock-out newborn mice (Rohr et al. 2002. J Clin Invest 109:1073-82).
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Biomaterial provider |
Ralph Witzgall
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Treatment protocol |
On the day of birth, offspring was killed, kidneys were removed and snap-frozen in liquid nitrogen. Total RNA was isolated from a half kidney of three wild-type homozygous and three Lmx1b-/- homozygous mice for microarray analyses.
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Growth protocol |
On the day of birth, offspring was killed, kidneys were removed and snap-frozen in liquid nitrogen. Total RNA was isolated from a half kidney of three wild-type homozygous and three Lmx1b-/- homozygous mice for microarray analyses.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNAs were isolated using the Nucleospin RNA II kit (Macherey-Nagel), including an on-column DNase I treatment to eliminate genomic DNA contamination, following the manufacturer's recommendations.
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Label |
Cy3
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Label protocol |
DNase-treated total RNA (1 µg) was synthesized into Cyanine 3 labeled cRNA (One-Color Microarray-Based Gene Expression Analysis Protocol, version 1.0.1, Agilent Technologies).
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Hybridization protocol |
Labeled cRNA (1.5 µg) from each sample was hybridized onto Agilent Whole Mouse Genome 4x44K Expression Arrays and washed according to manufacturer’s instructions.
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Scan protocol |
Fluorescent signals were measured using an Agilent DNA Microarray Scanner according to manufacturer’s instructions. Microarray data was extracted using Agilent Feature Extraction Software.
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Description |
Reference sample for the microarray analysis (wild type mouse), replicate 1.
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Data processing |
Significance analysis was performed with ArrayAssist Software (Agilent). Transcripts showing a fold change above 2-fold and a p-value below 0.05 were considered as significantly regulated.
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Submission date |
Jul 09, 2008 |
Last update date |
Dec 04, 2008 |
Contact name |
Anne Rascle |
E-mail(s) |
anne.rascle@vkl.uni-regensburg.de
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Phone |
+49 (0)941 2806598
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Organization name |
University of Regensburg
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Street address |
Universitaetsstrasse
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City |
Regensburg |
ZIP/Postal code |
93053 |
Country |
Germany |
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Platform ID |
GPL4134 |
Series (1) |
GSE12008 |
Identification of LMX1B target genes in a tet-off inducible HeLa cell line and in the mouse kidney |
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