|
| Status |
Public on Dec 04, 2008 |
| Title |
Kidney_Lmx1b null mouse_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Kidney from Lmx1b conventional knockout mouse
|
| Organism |
Mus musculus |
| Characteristics |
Kidney isolated from wild type and Lmx1b conventional knock-out newborn mice (Rohr et al. 2002. J Clin Invest 109:1073-82).
|
| Biomaterial provider |
Ralph Witzgall
|
| Treatment protocol |
On the day of birth, offspring was killed, kidneys were removed and snap-frozen in liquid nitrogen. Total RNA was isolated from a half kidney of three wild-type homozygous and three Lmx1b-/- homozygous mice for microarray analyses.
|
| Growth protocol |
On the day of birth, offspring was killed, kidneys were removed and snap-frozen in liquid nitrogen. Total RNA was isolated from a half kidney of three wild-type homozygous and three Lmx1b-/- homozygous mice for microarray analyses.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were isolated using the Nucleospin RNA II kit (Macherey-Nagel), including an on-column DNase I treatment to eliminate genomic DNA contamination, following the manufacturer's recommendations.
|
| Label |
Cy3
|
| Label protocol |
DNase-treated total RNA (1 µg) was synthesized into Cyanine 3 labeled cRNA (One-Color Microarray-Based Gene Expression Analysis Protocol, version 1.0.1, Agilent Technologies).
|
| |
|
| Hybridization protocol |
Labeled cRNA (1.5 µg) from each sample was hybridized onto Agilent Whole Mouse Genome 4x44K Expression Arrays and washed according to manufacturer’s instructions.
|
| Scan protocol |
Fluorescent signals were measured using an Agilent DNA Microarray Scanner according to manufacturer’s instructions. Microarray data was extracted using Agilent Feature Extraction Software.
|
| Description |
Test sample for the microarray analysis (Lmx1b null mouse), replicate 3.
|
| Data processing |
Significance analysis was performed with ArrayAssist Software (Agilent). Transcripts showing a fold change above 2-fold and a p-value below 0.05 were considered as significantly regulated.
|
| |
|
| Submission date |
Jul 09, 2008 |
| Last update date |
Dec 04, 2008 |
| Contact name |
Anne Rascle |
| E-mail(s) |
anne.rascle@vkl.uni-regensburg.de
|
| Phone |
+49 (0)941 2806598
|
| Organization name |
University of Regensburg
|
| Street address |
Universitaetsstrasse
|
| City |
Regensburg |
| ZIP/Postal code |
93053 |
| Country |
Germany |
| |
|
| Platform ID |
GPL4134 |
| Series (1) |
| GSE12008 |
Identification of LMX1B target genes in a tet-off inducible HeLa cell line and in the mouse kidney |
|
