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Sample GSM30459

Query DataSets for GSM30459

Status

Public on Sep 15, 2004

Title

normal monocyte 1

Sample type

RNA

Source name

normal CD14+ cells from peripheral blood

Organism

Homo sapiens

Extracted molecule

total RNA

Description

CD14+ cells were isolated from normal peripheral blood mononuclear cells by Dako Tuek4 antibody and Rat anti-mouse IgG2a+b magnetic bead selection to >95% purity. Total RNA was isolated with Qiagen RNeasy columns and converted to biotin-labeled cRNA by standard Affymetrix protocols. Targets were produced using standard Affymetrix procedures from about 12ug of total RNA. For analysis with MAS5 the sample was scaled to an arbitrary value of 1000 by overall hybridization intensity to allow comparison of multiple samples.
Keywords = monocyte, CD14

Submission date

Sep 10, 2004

Last update date

May 27, 2005

Contact name

Lynn Graf

E-mail(s)

lgraf@fhcrc.org

Phone

206-667-4545

Fax

206-667-5978

URL

http://none

Organization name

Fred Hutchinson Cancer Research Center

Department

Clinical Research Division

Lab

Torok-Storb

Street address

1100 Fairview Ave. N. D1-100

City

Seattle

State/province

ZIP/Postal code

98109

Country

USA

Platform ID

GPL80

Series (1)

GSE1746

CD14+ cell analysis

Data table header descriptions
ID_REF
VALUE	'signal', a measure of the abundance of a transcript
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A) or marginal (M)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
A28102_at	475	P	0.021902
AB000114_at	241.3	P	0.013811
AB000115_at	960.9	P	0.002556
AB000220_at	517.9	P	0.000081
AB000381_s_at	8.2	A	0.712257
AB000409_at	1583.7	P	0.000258
AB000410_s_at	165.9	A	0.659339
AB000449_at	383.3	P	0.010317
AB000450_at	607.7	P	0.004017
AB000460_at	1959.5	P	0.002867
AB000462_at	433.6	A	0.083592
AB000464_at	801.3	P	0.009337
AB000466_at	94.2	A	0.978098
AB000467_at	142.2	A	0.960339
AB000468_at	1529	P	0.000225
AB000584_at	739.1	A	0.368438
AB000816_s_at	54.8	A	0.712257
AB000895_at	166.2	A	0.868639
AB000896_at	358.8	A	0.382599
AB000897_at	144.9	A	0.470241

Total number of rows: 7129

Table truncated, full table size 194 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM30459.cel.gz	2.2 Mb	(ftp)(http)	CEL