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Sample GSM30460

Query DataSets for GSM30460

Status

Public on Sep 15, 2004

Title

normal monocyte 2

Sample type

RNA

Source name

normal CD14+ cells from peripheral blood

Organism

Homo sapiens

Extracted molecule

total RNA

Description

CD14+ cells were isolated from normal peripheral blood mononuclear cells by Dako Tuek4 antibody and Rat anti-mouse IgG2a+b magnetic bead selection to >95% purity. Total RNA was isolated with Qiagen RNeasy columns and converted to biotin-labeled cRNA by standard Affymetrix protocols. Targets were produced using standard Affymetrix procedures from about 12ug of total RNA. For analysis with MAS5 the sample was scaled to an arbitrary value of 1000 by overall hybridization intensity to allow comparison of multiple samples.
Keywords = monocyte, CD14

Submission date

Sep 10, 2004

Last update date

May 27, 2005

Contact name

Lynn Graf

E-mail(s)

lgraf@fhcrc.org

Phone

206-667-4545

Fax

206-667-5978

URL

http://none

Organization name

Fred Hutchinson Cancer Research Center

Department

Clinical Research Division

Lab

Torok-Storb

Street address

1100 Fairview Ave. N. D1-100

City

Seattle

State/province

ZIP/Postal code

98109

Country

USA

Platform ID

GPL80

Series (1)

GSE1746

CD14+ cell analysis

Data table header descriptions
ID_REF
VALUE	'signal', a measure of the abundance of a transcript
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A) or marginal (M)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
A28102_at	286.9	A	0.139482
AB000114_at	104.8	A	0.216524
AB000115_at	579.4	P	0.023929
AB000220_at	316.6	P	0.000446
AB000381_s_at	32.8	A	0.672921
AB000409_at	1402.4	P	0.000509
AB000410_s_at	149.6	A	0.645547
AB000449_at	373.9	P	0.013811
AB000450_at	844.1	P	0.020022
AB000460_at	2725.5	P	0.000754
AB000462_at	340.3	A	0.227636
AB000464_at	765.3	P	0.010317
AB000466_at	134.5	A	0.910522
AB000467_at	609.7	A	0.323735
AB000468_at	2880.2	P	0.000509
AB000584_at	825.3	A	0.368438
AB000816_s_at	76.9	A	0.544587
AB000895_at	207.6	A	0.420267
AB000896_at	379.8	A	0.368438
AB000897_at	59.7	A	0.772364

Total number of rows: 7129

Table truncated, full table size 194 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM30460.cel.gz	2.4 Mb	(ftp)(http)	CEL