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Sample GSM30463

Query DataSets for GSM30463

Status

Public on Sep 15, 2004

Title

normal monocyte 5

Sample type

RNA

Source name

normal CD14+ cells from peripheral blood

Organism

Homo sapiens

Extracted molecule

total RNA

Description

CD14+ cells were isolated from normal peripheral blood mononuclear cells by Dako Tuek4 antibody and Rat anti-mouse IgG2a+b magnetic bead selection to >95% purity. Total RNA was isolated with Qiagen RNeasy columns and converted to biotin-labeled cRNA by standard Affymetrix protocols. Targets were produced using standard Affymetrix procedures from about 12ug of total RNA. For analysis with MAS5 the sample was scaled to an arbitrary value of 1000 by overall hybridization intensity to allow comparison of multiple samples.
Keywords = monocyte, CD14

Submission date

Sep 10, 2004

Last update date

May 27, 2005

Contact name

Lynn Graf

E-mail(s)

lgraf@fhcrc.org

Phone

206-667-4545

Fax

206-667-5978

URL

http://none

Organization name

Fred Hutchinson Cancer Research Center

Department

Clinical Research Division

Lab

Torok-Storb

Street address

1100 Fairview Ave. N. D1-100

City

Seattle

State/province

ZIP/Postal code

98109

Country

USA

Platform ID

GPL80

Series (1)

GSE1746

CD14+ cell analysis

Data table header descriptions
ID_REF
VALUE	'signal', a measure of the abundance of a transcript
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A) or marginal (M)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
A28102_at	366.3	P	0.018281
AB000114_at	94.1	P	0.018281
AB000115_at	569.3	P	0.003595
AB000220_at	199.8	P	0.000225
AB000381_s_at	17.2	A	0.724854
AB000409_at	1722.6	P	0.000225
AB000410_s_at	49.4	A	0.883887
AB000449_at	206.4	P	0.001248
AB000450_at	539.9	P	0.001796
AB000460_at	1594.3	P	0.000147
AB000462_at	320.2	P	0.015183
AB000464_at	336.3	P	0.01667
AB000466_at	82.9	A	0.953518
AB000467_at	311.3	A	0.574038
AB000468_at	1550.7	P	0.00006
AB000584_at	531.2	A	0.368438
AB000816_s_at	158	A	0.139482
AB000895_at	73	A	0.712257
AB000896_at	224.4	A	0.39692
AB000897_at	143.8	A	0.327079

Total number of rows: 7129

Table truncated, full table size 193 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM30463.cel.gz	2.3 Mb	(ftp)(http)	CEL