|
| Status |
Public on Mar 01, 2019 |
| Title |
siERK3 1 |
| Sample type |
RNA |
| |
|
| Source name |
A375 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A375
tissue: melanoma
sirna target: ERK3
|
| Treatment protocol |
DharmaFECT Transfection Reagent (Dharmacon) was used to transiently knock down mRNA following the manufacturer’s instructions. siRNA used were: siERK3 (Ambion Silencer® Select Pre-Designed siRNA); and Ambion® Silencer® Select Negative Control siRNA).
|
| Growth protocol |
A375 cells were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% antibiotics penicillin-streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA of each cell line was extracted using Trizol® reagent (Ambion).
|
| Label |
biotin
|
| Label protocol |
As per manufacturer (Afftmetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized with Human Clariom S microarrays (Affymetrix) and scanned at the WSU Center for Genomics Research Core Facility.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000 according to the manufacturer's instruction (Affymetrix).
|
| Data processing |
The array CEL files were acquired using Affymetrix Command Console (AGCC version 4.1). CHP files were generated using Affymetrix Transcriptome Analysis Software v4.0.
|
| |
|
| Submission date |
Mar 19, 2018 |
| Last update date |
Mar 01, 2019 |
| Contact name |
Michael Markey |
| E-mail(s) |
michael.markey@wright.edu
|
| Phone |
937-775-4536
|
| Fax |
937-775-3031
|
| URL |
http://www.med.wright.edu/cgr/
|
| Organization name |
Wright State Unviersity
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Center for Genomics Research
|
| Street address |
3640 Colonel Glenn Hwy.
|
| City |
Dayton |
| State/province |
OH |
| ZIP/Postal code |
45435 |
| Country |
USA |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE112040 |
Gene expression changes in A375 melanoma cells in response to ERK3 knockdown. |
|
