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Sample GSM305583 Query DataSets for GSM305583
Status Public on Jan 08, 2009
Title AJC A36
Sample type RNA
 
Source name hepatic tissue, A/J, prenatal control
Organism Mus musculus
Characteristics male 7month old offspring
Treatment protocol pregnant mice were either treated as controls (C) and fed ad libitum for the duration of pregnancy or were treated as restricted (R) and subjected to undernutrition restriction diet. Whole mouse livers were extracted from 27 week old offspring from C or R treated mothers and flash frozen in liquid nitrogen.
Extracted molecule total RNA
Extraction protocol Total Hepatic RNA was extracted from the frozen tissue using RNeasy Mini Kits with on-column RNase-free DNase Kit digestion (Quigen)
Label Biotin
Label protocol standard protocols for Affymetrix GeneChip® Mouse Gene 1.0 ST Array
 
Hybridization protocol standard protocols for Affymetrix GeneChip® Mouse Gene 1.0 ST Array
Scan protocol standard protocols for Affymetrix GeneChip™3000 7G laser confocal slide scanner
Description no additional information
Data processing standard protocols for Affymetrix GeneChip® Mouse Gene 1.0 ST Array using Affymetrix Expression Console software
 
Submission date Jul 15, 2008
Last update date Dec 29, 2008
Contact name brian knight
E-mail(s) bknight@mshri.on.ca
Phone 4165868224
Organization name samuel lunenfeld research institute
Street address 25 orde st
City toronto
ZIP/Postal code m5s3a6
Country Canada
 
Platform ID GPL6246
Series (1)
GSE12117 Role of inherent strain differences in prenatally induced differential metabolic syndrome phenotype and gene expression

Data table header descriptions
ID_REF
VALUE signal intensity in Affymetrix Expression Console was normalized using RMA algorithm

Data table
ID_REF VALUE
10338001 11.79768
10338003 10.24057
10338004 9.056211
10338017 12.72769
10338025 8.99302
10338026 13.0884
10338029 9.67944
10338035 8.810434
10338036 9.36664
10338037 3.309309
10338041 10.63448
10338042 10.13304
10338044 11.63682
10338047 6.329066
10338056 1.918974
10338059 13.11017
10338060 4.153519
10338063 2.379264
10338064 5.900204
10338065 6.297572

Total number of rows: 35512

Table truncated, full table size 620 Kbytes.




Supplementary file Size Download File type/resource
GSM305583.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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