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Sample GSM3082716 Query DataSets for GSM3082716
Status Public on Jun 04, 2020
Title U87NC_day7 rep3
Sample type SRA
 
Source name U87 cell
Organism Homo sapiens
Characteristics cell type: glioblastoma cells
cell line: U87
genotype: U87 cells transduced with lentivirus encoding vector
Treatment protocol Cells were treated with 350 µM TMZ for 7 and 14 days.
Growth protocol The cells were routinely maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) with 10% heat-inactivated fetal bovine serum (FBS, HyClone, Logan, UT, USA) in a humidified 5% CO2 atmosphere at 37℃.
Extracted molecule total RNA
Extraction protocol RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model BGISEQ-500
 
Data processing SOAPnuke(v1.5.2) software used for basecalling.
RNA-seq reads were mapped to hg19 whole genome by Hisat2 software.
DEseq2 method was used to perform differentially expressed gene analysis.
Genome_build: hg19
Supplementary_files_format_and_content: excel format files include FPKM values
 
Submission date Apr 05, 2018
Last update date Jun 04, 2020
Contact name Kai Huang
E-mail(s) huangkai@tijmu.edu.cn
Phone 13752390609
Organization name tianjin medical university
Street address anshan roud
City tianjin
ZIP/Postal code 300054
Country China
 
Platform ID GPL23227
Series (2)
GSE112734 A genome-wide screen identifies E2F6 as critical factor for TMZ chemoresistance in EGFRvIII expressing GBM (CRISPR library RNA-seq)
GSE112736 A genome-wide screen identifies E2F6 as critical factor for TMZ chemoresistance in EGFRvIII expressing GBM
Relations
BioSample SAMN08868540
SRA SRX3889241

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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