|
| Status |
Public on Jan 01, 2009 |
| Title |
DF 2 h (Cy5) vs. 0 h (Cy3) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Dermal fibroblasts, IL-1-treated (2 h)
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6
|
| Treatment protocol |
Cells were incubated with 20 ng/ml IL-1 for 0, 1, 2, or 4 h.
|
| Growth protocol |
Mouse dermal fibroblasts were cultured in DMEM medium containing antibiotics and 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
Fluorescent cRNA was generated from 5 µg of total RNA using Agilent Two-Color Quick Amp Labeling Kit. The reaction and purification conditions were based on the manufacturer's protocol.
|
| |
|
| Channel 2 |
| Source name |
Dermal fibroblasts, untreated (0 h)
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6
|
| Treatment protocol |
Cells were incubated with 20 ng/ml IL-1 for 0, 1, 2, or 4 h.
|
| Growth protocol |
Mouse dermal fibroblasts were cultured in DMEM medium containing antibiotics and 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Fluorescent cRNA was generated from 5 µg of total RNA using Agilent Two-Color Quick Amp Labeling Kit. The reaction and purification conditions were based on the manufacturer's protocol.
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequentially with GE Wash Buffer 1 at room temperature and GE Wash Buffer 2 at 31°C.
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner. Images were quantified using Agilent Feature Extraction Software (version A.7.5).
|
| Description |
Replicate 1 of 2
|
| Data processing |
LOWESS normalized, background-subtracted data obtained from log2 of processed test signal/processed reference signal. Agilent software was used.
|
| |
|
| Submission date |
Jul 29, 2008 |
| Last update date |
Jul 30, 2008 |
| Contact name |
Jin Mo Park |
| E-mail(s) |
jmpark@cbrc2.mgh.harvard.edu
|
| Phone |
617-643-2328
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Cutaneous Biology Research Center
|
| Street address |
149 Thirteenth Street
|
| City |
Charlestown |
| State/province |
MA |
| ZIP/Postal code |
02129 |
| Country |
USA |
| |
|
| Platform ID |
GPL2872 |
| Series (1) |
| GSE12285 |
Interleukin-1-induced gene expression in mouse dermal fibroblasts |
|
